1. Detecting Psychoactive Drugs in the Developmental Stages of Psilocybe cyanescencs Gross, Susan T. J Forensic Sci 2000;45(3): 527–537.

2. Why this is important Clandestine Grow Operations Clandestine Grow Operations Law Enforcement Personnel Law Enforcement Personnel Forensic Chemists Forensic Chemists

3. Introduction Psilocybe Cyanescens Psilocybe Cyanescens Psilocybin/Psilocin Psilocybin/Psilocin Various Stages of Growth Various Stages of Growth

4. Methods Mushrooms grown from spore stage in sterile conditions. Mushrooms grown from spore stage in sterile conditions. Some were grown in dark and some in light Some were grown in dark and some in light TLC of various extracts TLC of various extracts GC/MS of various extracts GC/MS of various extracts

5. Preparation of Samples Standard Psilocybin and Psilocin obtained Standard Psilocybin and Psilocin obtained Mycelium Knot Samples soaked in MeOH Mycelium Knot Samples soaked in MeOH All other Extracts cleaned w/ H2SO4, washed with Chloroform. Made Basic, psilocybin/psilocin extracted with Chloroform, and reconstituted with MeOH All other Extracts cleaned w/ H2SO4, washed with Chloroform. Made Basic, psilocybin/psilocin extracted with Chloroform, and reconstituted with MeOH

6. Results

7. Results

8. Conclusions Psychoactive Drugs weren’t detected in spores or mycelium Psychoactive Drugs weren’t detected in spores or mycelium Both Psilocybin and Psilocin were detected in mycelium knots and the fruiting body of the mushroom Both Psilocybin and Psilocin were detected in mycelium knots and the fruiting body of the mushroom Light effects growth of P. cyanescens but not detection or content of psychoactives Light effects growth of P. cyanescens but not detection or content of psychoactives