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1 Cellular Division
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2 Cell Division All cells are derived from pre- existing cells All cells are derived from pre- existing cells New cells are produced for growth and to replace damaged or old cells New cells are produced for growth and to replace damaged or old cells
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3 Keeping Cells Identical The instructions for making cell parts are encoded in the DNA, so each new cell must get a complete set of the DNA molecules
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4 DNA Replication DNA must be copied or replicated before cell division DNA must be copied or replicated before cell division Each new cell will then have an identical copy of the DNA Each new cell will then have an identical copy of the DNA Original DNA strand Two new, identical DNA strands
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5 Identical Daughter Cells Parent Cell Two identical daughter cells
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6 Chromosomes
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7 Eukaryotic Chromosomes All eukaryotic cells store genetic information in chromosomes All eukaryotic cells store genetic information in chromosomes Human body cells have 46 chromosomes or 23 identical pairs Human body cells have 46 chromosomes or 23 identical pairs Do you think the number chromosomes determines how how intelligent you are? Do you think the number chromosomes determines how how intelligent you are?
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8 Eukaryotic Chromosomes Each chromosome is composed of a single, tightly coiled DNA molecule Each chromosome is composed of a single, tightly coiled DNA molecule Chromosomes can’t be seen when cells aren’t dividing and are called chromatin Chromosomes can’t be seen when cells aren’t dividing and are called chromatin
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9 Compacting DNA into Chromosomes DNA is tightly coiled around proteins called histones
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10 Chromosomes in Dividing Cells Duplicated chromosomes are called sister chromatids & are held together by the centromere Called Sister Chromatids
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11 Karyotype A picture of the chromosomes from a human cell arranged in pairs by size A picture of the chromosomes from a human cell arranged in pairs by size First 22 pairs are called autosomes First 22 pairs are called autosomes Last pair are the sex chromosomes Last pair are the sex chromosomes XX female or XY male XX female or XY male
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12 Boy or Girl? Y - Chromosome X - Chromosome The Y Chromosome Decides
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13 Cell Reproduction
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14 Types of Cell Reproduction Asexual reproduction involves a single cell dividing to make 2 new, identical daughter cells (an exact copy of parent cell) Asexual reproduction involves a single cell dividing to make 2 new, identical daughter cells (an exact copy of parent cell) EX: Mitosis EX: Mitosis Sexual reproduction involves two cells (egg & sperm) joining to make a new cell (zygote) that is NOT identical to the original cells Sexual reproduction involves two cells (egg & sperm) joining to make a new cell (zygote) that is NOT identical to the original cells EX: MEIOSIS EX: MEIOSIS
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15 The Cell Cycle
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16 Five Phases of the Cell Cycle G 1 - primary growth phase G 1 - primary growth phase S – synthesis; DNA replicated S – synthesis; DNA replicated G 2 - secondary growth phase G 2 - secondary growth phase collectively these 3 stages are called interphase M - mitosis M - mitosis C - cytokinesis C - cytokinesis
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17 Cell Cycle
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18 Interphase – S Stage Synthesis stage Synthesis stage DNA is copied or replicated DNA is copied or replicated Two identical copies of DNA Original DNA
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19 What’s Happening in Interphase? What the cell looks like Animal Cell What’s occurring
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20 Sketch the Cell Cycle Daughter Cells DNA Copied Cells Mature Cells prepare for Division Cell Divides into Identical cells
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21 Mitosis
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22 Mitosis Division of the nucleus Division of the nucleus Has four stages Has four stages Doesn’t occur in some cells such as brain cells Doesn’t occur in some cells such as brain cells
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23 Four Mitotic Stages Prophase Prophase Metaphase Metaphase Anaphase Anaphase Telophase Telophase
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24 Early Prophase Chromatin in nucleus condenses to form visible chromosomes Chromatin in nucleus condenses to form visible chromosomes Chromosomes Nucleolus Cytoplasm Nuclear Membrane
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25 Late Prophase Nuclear membrane & nucleolus are broken down Nuclear membrane & nucleolus are broken down Chromosomes continue condensing & are clearly visible Chromosomes continue condensing & are clearly visible Spindle finishes forming between the poles of the cell Spindle finishes forming between the poles of the cell
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26 Late Prophase Nucleus & Nucleolus have disintegrated Chromosomes
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27 Sketch The Spindle
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28 Metaphase Chromosomes, attached to the spindle apparatus, move to the center of the cell Chromosomes, attached to the spindle apparatus, move to the center of the cell Chromosomes are now lined up at the equator Chromosomes are now lined up at the equator Pole of the Cell Equator of Cell
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29 Metaphase Chromosomes lined at the Equator Asters at the poles Spindle Fibers
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30 Metaphase Aster Chromosomes at Equator
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31 Anaphase Occurs rapidly Occurs rapidly Sister chromatids are pulled apart to opposite poles of the cell by spindles (microtubules) Sister chromatids are pulled apart to opposite poles of the cell by spindles (microtubules)
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32 Anaphase Sister Chromatids being separated
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33 Telophase Sister chromatids at opposite poles Sister chromatids at opposite poles CYTOKINESIS occurs CYTOKINESIS occurs Chromosomes reappear as chromatin Chromosomes reappear as chromatin
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34 Comparison of Anaphase & Telophase
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35 Cytokinesis Means division of the cytoplasm Means division of the cytoplasm Division of cell into two, identical halves called daughter cells Division of cell into two, identical halves called daughter cells In plant cells, cell plate forms at the equator to divide cell In plant cells, cell plate forms at the equator to divide cell In animal cells, cleavage furrow forms to split cell In animal cells, cleavage furrow forms to split cell
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36 Cytokinesis Cleavage furrow in animal cell Cell plate in plant cell
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37 Mitotic Stages
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38 Daughter Cells of Mitosis Have the same number of chromosomes as each other and as the parent cell from which they were formed Have the same number of chromosomes as each other and as the parent cell from which they were formed Identical to each other, but smaller than parent cell Identical to each other, but smaller than parent cell Must grow in size to become mature cells (G 1 of Interphase) Must grow in size to become mature cells (G 1 of Interphase)
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39 Identical Daughter Cells Chromosome number the same, but cells smaller than parent cell What is the 2n or diploid number? 2
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40 Review of Mitosis
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41 Interphase Prophase Metaphase Anaphase Telophase Name the Mitotic Stages: Name this?
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42 Eukaryotic Cell Division Used for growth and repair Used for growth and repair Produce two new cells identical to the original cell Produce two new cells identical to the original cell Cells are diploid (2n) Cells are diploid (2n) Chromosomes during Metaphase of mitosis ProphaseMetaphase Anaphase Telophase Cytokinesis
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43 Mitosis Animation Name each stage as you see it occur?
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44 Mitosis in Onion Root Tips Do you see any stages of mitosis?
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45 Draw & Learn these Stages
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46 Draw & Learn these Stages
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47 Test Yourself over Mitosis
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48 Mitosis Quiz
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49 Mitosis Quiz
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50 Name the Stages of Mitosis: Interphase Early prophase Mid-Prophase Late Prophase Metaphase Late Anaphase Early Anaphase Early Telophase, Begin cytokinesis Late telophase, Advanced cytokinesis
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51 Identify the Stages Early, Middle, & Late Prophase Late Prophase MetaphaseAnaphase Late Anaphase Telophase Telophase & Cytokinesis ? ? ?? ? ??
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52 Locate the Four Mitotic Stages in Plants Metaphase Prophase Anaphase Telophase
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53 Uncontrolled Mitosis If mitosis is not controlled, unlimited cell division occurs causing cancerous tumors If mitosis is not controlled, unlimited cell division occurs causing cancerous tumors Oncogenes are special proteins increase the chance that a normal cell develops into a tumor cell Oncogenes are special proteins that increase the chance that a normal cell develops into a tumor cell Cancer cells
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54 Meiosis Formation of Gametes (Eggs & Sperm)
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55 Facts About Meiosis Preceded by interphase which includes chromosome replication Preceded by interphase which includes chromosome replication Two meiotic divisions --- Meiosis I and Meiosis II Two meiotic divisions --- Meiosis I and Meiosis II Called Reduction- division Called Reduction- division Original cell is diploid (2n) Original cell is diploid (2n) Four daughter cells produced that are monoploid (1n) Four daughter cells produced that are monoploid (1n)
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56 Facts About Meiosis Daughter cells contain half the number of chromosomes as the original cell Daughter cells contain half the number of chromosomes as the original cell Produces gametes (eggs & sperm) Produces gametes (eggs & sperm) Occurs in the testes in males (Spermatogenesis) Occurs in the testes in males (Spermatogenesis) Occurs in the ovaries in females (Oogenesis) Occurs in the ovaries in females (Oogenesis)
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57 Start with 46 double stranded chromosomes (2n) After 1 division - 23 double stranded chromosomes (n) After 1 division - 23 double stranded chromosomes (n) After 2nd division - 23 single stranded chromosomes (n) After 2nd division - 23 single stranded chromosomes (n) Occurs in our germ cells that produce gametes More Meiosis Facts
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58 Why Do we Need Meiosis? It is the fundamental basis of sexual reproduction It is the fundamental basis of sexual reproduction Two haploid (1n) gametes are brought together through fertilization to form a diploid (2n) zygote Two haploid (1n) gametes are brought together through fertilization to form a diploid (2n) zygote
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59 Fertilization – “Putting it all together” 1n =3 2n = 6
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60 Replication of Chromosomes Replication is the process of duplicating a chromosome Replication is the process of duplicating a chromosome Occurs prior to division Occurs prior to division Replicated copies are called sister chromatids Replicated copies are called sister chromatids Held together at centromere Held together at centromere Occurs in Interphase
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61 A Replicated Chromosome Homologs Homologs (same genes, different alleles) (same genes, different alleles) Sister Chromatids (same genes, same alleles) Gene X Homologs separate in meiosis I and therefore different alleles separate.
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62 Meiosis Forms Haploid Gametes Meiosis must reduce the chromosome number by half Meiosis must reduce the chromosome number by half Fertilization then restores the 2n number Fertilization then restores the 2n number from momfrom dadchild meiosis reduces genetic content too much! The right number!
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63 Meiosis: Two Part Cell Division Homologsseparate Sisterchromatidsseparate Diploid Meiosis I Meiosis II Diploid Haploid
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64 Meiosis I: Reduction Division Nucleus Spindlefibers Nuclearenvelope Early Prophase I (Chromosome number doubled) Late Prophase I Metaphase I Anaphase I Telophase I (diploid)
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65 Prophase I Early prophase Homologs pair. Homologs pair. Crossing over occurs Crossing over occurs. Late prophase Chromosomes condense. Chromosomes condense. Spindle forms. Spindle forms. Nuclear envelope fragments. Nuclear envelope fragments.
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66 Tetrads Form in Prophase I Homologous chromosomes (each with sister chromatids) Homologous chromosomes (each with sister chromatids) Join to form a TETRAD Called Synapsis
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67 Crossing-Over Homologous chromosomes in a tetrad cross over each other Homologous chromosomes in a tetrad cross over each other Pieces of chromosomes or genes are exchanged Pieces of chromosomes or genes are exchanged Produces Genetic recombination in the offspring Produces Genetic recombination in the offspring
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68 Homologous Chromosomes During Crossing-Over
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69 Crossing-over multiplies the already huge number of different gamete types produced by independent assortment Crossing-Over
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70 Metaphase I Homologous pairs of chromosomes align along the equator of the cell
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71 Anaphase I Homologs separate and move to opposite poles. Sister chromatids remain attached at their centromeres attached at their centromeres.
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72 Telophase I Nuclear envelopes reassemble. Spindle disappears. Cytokinesis divides cell into two.
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73 Meiosis II Only one homolog of each chromosome is present in the cell Only one homolog of each chromosome is present in the cell. Meiosis II produces gametes with one copy of each chromosome and thus one copy of each gene. Sister chromatids carry identical genetic information. Gene X
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74 Meiosis II: Reducing Chromosome Number Prophase II Metaphase II Anaphase II Telophase II 4 Identical haploid cells
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75 Prophase II Nuclear envelope fragments. Spindle forms.
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76 Metaphase II Chromosomes align along equator of cell.
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77 Anaphase II Sister chromatids separate and move to opposite poles Sister chromatids separate and move to opposite poles. Equator Pole
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78 Telophase II Nuclear envelope assembles. Chromosomes decondense. Spindle disappears. Cytokinesis divides cell into two.
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79 Results of Meiosis Gametes (egg & sperm) form Four haploid cells with one copy of each chromosome
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80 Gametogenesis Oogenesis or Spermatogenesis
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81 Spermatogenesis Occurs in the testes Occurs in the testes Two divisions produce 4 spermatids Two divisions produce 4 spermatids Spermatids mature into sperm Spermatids mature into sperm Men produce about 250,000,000 sperm per day Men produce about 250,000,000 sperm per day
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82 Spermatogenesis in the Testes Spermatid
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83Spermatogenesis
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84 Oogenesis Occurs in the ovaries Occurs in the ovaries Two divisions produce 3 polar bodies that die and 1 egg Two divisions produce 3 polar bodies that die and 1 egg Polar bodies die because of unequal division of cytoplasm Polar bodies die because of unequal division of cytoplasm Immature egg called oocyte Immature egg called oocyte Starting at puberty, one oocyte matures into an ovum (egg) every 28 days Starting at puberty, one oocyte matures into an ovum (egg) every 28 days
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85 Oogenesis in the Ovaries
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86 Oogenesis Oogonium(diploid) Mitosis Primaryoocyte(diploid) Meiosis I Secondaryoocyte(haploid) Meiosis II (if fertilization occurs) First polar body may divide (haploid)Polarbodiesdie Ovum (egg) Second polar body (haploid) a A X X a X A X a X a X Matureegg A X A X
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87 Comparing Mitosis and Meiosis
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88 MitosisMeiosis Number of divisions 12 Number of daughter cells 24 Genetically identical? YesNo Chromosome # Same as parent Half of parent Where Somatic cells Germ cells When Throughout life At sexual maturity Role Growth and repair Sexual reproduction Comparison of Divisions
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