1. Supplemental Fig. S1 extracellular (P=0.000) cell wall (P=0.000) ribosome (P=0.001) ER (P=0.294) golgi apparatus (P=0.005) plasma membrane (P=0.000) mitochondria (P=0.000) cytosol (P=0.553) other cellular components (P=0.000) nucleus (P=0.000) other membranes (P=0.970) plastid (P=0.000) other cytoplasmic components (P=0.383) unknown cellular components(P=0.520) chloroplast (P=0.000) other intracellular components (P=0.000) A. Cellular component Brassica COS A. thaliana receptor binding or activity (P=0.049) structural molecule activity (P=0.207) transporter activity (P=0.000) other molecular functions (P=0.003) transcription factor activity(P=0.003) kinase activity (P=0.000) nucleic acid binding (P=0.292) nucleotide binding (P=0.307) DNA or RNA binding (P=0.777) transferase activity (P=0.000) other enzyme activity (P=0.020) hydrolase activity (P=0.063) other binding (P=0.001) protein binding (P=0.000) unknown molecular functions (P=0.000) B. Molecular function Brassica COS A. thaliana electron transport or energy pathways (P=0.176) signal transduction (P=0.000) transcription,DNA- dependent (P=0.632) transport (P=0.000) other biological processes (P=0.000) response to abiotic or biotic stimulus (P=0.000) protein metabolism (P=0.082) cell organization and biogenesis (P=0.592) DNA or RNA metabolism (P=0.000) response to stress (P=0.011) developmental processes (P=0.127) other metabolic processes (P=0.000) unknown biological processes (P=0.000) other cellular processes (P=0.000) C. Biological process Brassica COS A. thaliana

2. Supplemental Fig. S2 COS0015COS0084 AtRsABCABACBCA+BA+BA+CA+CB+CB+CAtRsABCABACBCA+BA+BA+CA+CB+CB+C COS0264COS0420 AtRsABCABACBCA+BA+BA+CA+CB+CB+C AtRsABCABACBCA+BA+BA+CA+CB+CB+C COS0566COS0842 AtRsABCABACBCA+BA+BA+CA+CB+CB+C AtRsABCABACBCA+BA+BA+CA+CB+CB+C

3. Supplemental Fig. S3 0.02 B. rupestris B. rapa B. macrocarpa B. bivoniana B. drepanensis B. villosa B. oleracea B. nigra R. sativus B. spinescens B. fruticulosa B. repanda A. thaliana 98 59 75 84 90 27 28 65 27

4. Supplemental Fig. S4 AK1 AK2 AK3 AK4 AK5 AK6 AK7 AK8

5. Supplemental Fig. S5. LF MF1 MF2 A. AK1

6. Supplemental Fig. S5 B. AK2 LF MF1 MF2

7. Supplemental Fig. S5 C. AK3 LF MF1 MF2

8. Supplemental Fig. S5 D. AK4 LF MF1 MF2

9. Supplemental Fig. S5 E. AK5 LF MF1 MF2

10. Supplemental Fig. S5 F. AK6 LF MF1 MF2

11. Supplemental Fig. S5 G. AK7 LF MF1 MF2

12. Supplemental Fig. S5 H. AK8 LF MF1 MF2