1. Biomat (LV, 20 Pa) Uncoated coccolith (LV, 20 Pa) JSM-7001F-LV - Applications

2. Tin oxide on tin Carbon nanotube JSM-7001F-LV - Applications

3. EBSD grain orientation map of Mo (Ti,Zr) alloy Fe L WDS map of polished thin section of ore JSM-7001F-LV - Applications

4. MultiBeam JIB-4500  FIB  Ion source Ga liquid metal ion  Acc. Voltage 1 to 30kV (in steps)  Magnification 30x (wide view) 100x to 300,000x  Max. beam current 30 nA (at 30kV)  SEM  Beam source LaB 6  Acc. Voltage 0.3 to 30 kV (in steps)  Magnification 5x to 300,000x  Max. beam current 1000 nA (at 30 kV)  Goniometer stage X: 76 mm; Y: 76 mm; Z: 5 to 48mm T: -10 to 90º; R: 360º endless  Vacuum pump SIP (x2), TMP/RP (x2)

5. Features and Functions of SEM/FIB Features High speed milling, large ion beam current High resolution ion column Simultaneous milling & image observation Bitmap milling for various shapes 3D view (S 3 TM ) High utility specimen chamber High performance SEM column with LaB 6 Confidential Functions 1.Progressive sectioning (slice and view) 2.Nanomachining 3.Site-specific preparation of TEM sections

6. Simultaneous Milling & Imaging Volkert et al, MRS Bulletin 32, May, 2007

7. FIB and Micromachining Mayer et al, MRS Bulletin 32, May, 2007

8. FIB and Micromachining Mayer et al, MRS Bulletin 32, May, 2007

9. Neuron cage (Meng) – 6490, SEI, 1 kV

10. Neuron cage (Meng) – Video

11. Emitter tip Tip radius less than 200nm Milling time : 2h Specimen : Si Bitmap Milling for Various Shapes Confidential

12. Types of Research Supported Biological and biomedical Bio and non-bio nanoscience Materials science / Chemistry / Physics Earth science / geo / geo-bio Electrical, mechanical, and civil engineering Physics Dental science Pharmacological

13. Access / Scheduling / Rates Open to all USC users Supported by two full-time staff plus post-doc Web-based sign-up at http://www.usc.edu/dept/CEMMA/cemma.html http://www.usc.edu/dept/CEMMA/cemma.html Rates (present) $45/hr self-use $75/hr with operator

14. Rates – EM Labs Nationwide “The median fee for internal and external users in academic organizations was $65 and $150, respectively. “ Source: Microscopy Today, July 2007 $150 $65

15. Break Even Capacity - BEC Assume 4 EMs, 2 techs, operating 8/5 and charging $50/hr Requires utilization of 52% to break even Source: Microscopy Today, July 2007

16. Summary Many thanks to Provost Much work left to do Stronger infrastructure to be built Comments and suggestions welcome