2. 分 子 病 理 實 驗 室 Molecular Pathology Lab 實驗室主持人：戚謹文, 周德盈 共同主持人 : 李芬瑤

3. The development of rational approaches to the diagnosis and treatment of cancer is dependent on the understanding of the mechanism that underlie the formation and progression of tumor

4. Traditional pathology Molecular pathology

5. Traditional pathology 1. Frozen section 2. Wax embedded tissue Hematoxylin-eosin stain Special stain: silver stain immunostain: vessel, estrogen receptors

6. Advances in molecular biology Genetic analyses Comparative genomic hybridization(CGH) Fluorescence in situ hybridization (FISH) Gene expression: transcription Genomic fingerprinting cDNA and oligonucleotide arrays Serial analysis of gene expression Differential display or representational difference analysis Gene expression: translation 2-D gels, HPLC, Mass spectrometry, tissue array, microdissection and laser microscopy

7. Molecular pathology (1) Human genome project 1. novel genes 2. known genes : amplification, mutation, deletion

9. Molecular pathology (2) Human genome project Studies of mRNA can determine the expression profile of a large number of transcripts.

11. Molecular pathology (3) Human genome project Proteomic methodologies can identify quantitative and qualitative protein changes in tumors, and to examine posttranslational modifications such as phosphorylation of proteins.

12. Loss of annexin 1 correlates with early onset of tumorigenesis in esophageal and prostate carcinoma. Paweletz et al. Cancer Res 60:6293, 2000

13. References: 1. Going and Gusterson Molecular pathology and future development. Eur J Cancer, 35:1895-1904, 1999. 2. Sirivatanauksorn et al. Laser-assisted microdissection: applications in molecular biology. J Pathology 189: 150-154, 1999.