1. Dissection Protocol for Gross Anatomy and Blood Supply
Heart Lab
Dissection Protocol for Gross Anatomy and Blood Supply

2. Dissection Instructions for Gross Anatomy
Make an incision with your scalpel three inches below the vibrissae on the ventral surface.
Cut through the muscle layers with the scalpel in a straight line down the sagittal plane to a point one inch anterior to the umbilicus.
Go back with the scissors and cut through the sternum.
At the area parallel with the forearms cut on the horizontal or transverse plane to the peripheral trunk. Follow the same procedure at the level of the diaphragm.
Stop – Observe - Record

4. Gross Anatomy Continued. . .
1.Carefully remove the pericardial membrane from the heart. Use a teasing needle and forceps to carefully dissect the muscle tissue and fatty tissue away from the major arteries and veins in the neck region. The arteries will appear red and the veins blue because the pig has been double injected with latex.
2.Carefully remove the heart by cutting the arteries and veins. Try to leave them in tact by cutting them anterior to their insertion into the heart.
Stop – Observe - Record

5. Supply
Observe the orientation of the blood supply coming into and leaving the heart.
Draw the heart in the ventral and dorsal view. This is a form of observation/data for biological sciences. This will allow you to follow blood supply. You could designate blood vessels with a B or R to indicate color.
Label: Superior and Inferior vena cava, rt.& lf. Atria, rt. & lf. Ventricle, Cardiac veins, Coronary arteries, Pulmonary arch, Aortic arch, rt. & lf. Pulmonary arteries and veins.

6. Dissection Instructions for Gross Anatomy Internal
Once the heart is removed, cut the heart in half along the frontal plane into dorsal and ventral halves.
Draw both halves. Identify and label the following structures: Rt. and Lf. Atria, Rt. and Lf. Ventricles, Tricuspid valve, Bicuspid (mitral) valve, Pulmonary trunk, Aorta, Vena Cava (inferior and superior), Interatrial septum, interventricular septum.
Stop – Observe – Record

8. Clean-Up Procedure Return Pigs to buckets.
Get paper towel, write your initials on them with a wax pencil, wet it, and wrap your heart in the paper. Give hearts to Sutton.
Clean pans and tools.
Return supplies to trays and return them to the closet in numerical order.
Put all trash (gloves, paper towel, etc.) in trash.