1. Lipid-dependent surface transport of the proton pumping
ATPase: A model to study plasma membrane biogenesis in yeast
Fan Mengdi Li Yandi

2. The Points Introduction
Biogenesis and transport of the proton pumping H+-ATPase

3. The general introduction of the
H+-ATPase in yeast
A good model to study plasma membrane biogenesis

4. The general introduction
The function of ATPase :
maintenance of the electrochemical gradient across the membrane
regulating intracellular PH
symporters

5. How did the H+-ATPase transport to the plasma membrane?
Begin: oligomerization and
package
Surface transport of the ATPase
Raft association
Stable integrate at the plasma membrane
protein
lipid

6. How did the H+-ATPase transport to the plasma membrane?
Every step needs the help of these lipids:
sterols
sphingolipids

7. Step 1: oligomerization
protein
Protein-lipid complex
lipid
C26
Pma1
The ER
In the ER, Pma1form a homo-oligomeric complex—the protein-lipid complex

8. Step 2: package Protein-lipid complex
coat protein complex II(a kind of transport vesicle)
Protein-lipid complex
The ER
The protein-lipid complex then packaged into a larger subclass of COPII

9. Step 3: modify of the Pma1 Protein-lipid complex Golgi body P-
coat protein complex II
Protein-lipid complex P-
The Pma1 becomes phosphorylated on multiple serine and threonine residues

10. Step 4: Pma1 transport to the cell surface
Golgi body
coat protein complex II
Protein-lipid complex
The lipid raft
C26
Pma1 is transported to the cell surface

11. How did the H+-ATPase transport to the plasma membrane?
Every step needs the help of these lipids:
sterols
sphingolipids

12. Step 5: Stable integrate at the plasma membrane
C26 H+
ATP
Lipid raft association

13. Two mutant of Pma1
Affect the stability, targeting, and detergent solubility during its transport to the surface

14. The first mutant:Pmal-7
Golgi body
coat protein complex II
Protein-lipid complex
Vacuole
Pmal-7:mistargeted from the Golgi to the vacuole without reaching the cell surface.

15. The second mutant:Pmal-10
Pmal-10: fails to become stabilized on the membrane, but to delivered to the vacuole, then degradation.
vacuole

16. Two mutant of Pma1
. The mutant indicate: lipid raft association may play important roles in maintaining protein stability at the plasma membrane.