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Published byBeverley Horton Modified over 9 years ago
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What invention helped with many discoveries in science?
Agenda for Thursday Sept 8th Microscopes Lab Learning Objectives Identify microscope parts and know their function Compute magnification for a microscope Measure the size (in microns) of objects under the microscope
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Basic Rules Carry using arm and base Flat Surface
Adjust light using diaphragm Use stage clips Focus with course adjustment on low and medium power only! High Power use fine adjustment only
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What is the function of the diaphragm on the microscope?
Agenda for Friday Sept 4th Microscope lab
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Magnification Total magnification = eyepiece magnification X objective magnification Eyepiece is 10x and objective is 10x what is total magnificaion?
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How to make a wet mount slide
Specimen on slide Drop of water/stain Cover slip on slide
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Calculate Field of View
Clear Ruler Determine how many millimeters across Convert to micrometers 1mm=1000μm So: if 1.5 mm across then field of view is 1.5mm x 1000 μm/1mm = 1500 μm
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Calculate Size of Specimen
Figure out how many will fit across the field of view (estimate) Divide field of view length by how many fit across If 8 fit across and field of view is 800 μm then specimen is 100 μm 800 μm/8 = 100 μm
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Remember! Carry using arm and base Flat Surface
Adjust light using diaphragm Use stage clips ONLY USE FINE ADJUSTMENT ON HIGH POWER!
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Objectives Identify microscope parts and know their function
Compute magnification for a microscope Measure the size (in microns) of objects under the microscope
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What is the total magnification if you have a 10x eyepiece and are using a 40x objective?
Agenda for Monday Sept 12th Microscope Quiz Microscope Lab Learning Objectives Identify microscope parts and know their function Compute magnification for a microscope Measure the size (in microns) of objects under the microscope
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Low power = 4000 μm Medium power = 1500 High power = 500
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