1. A UNIVERSAL PROGRAMMABLE TEMPERATURE SPRAY CHAMBER Jerry Dulude, Glass Expansion, jdulude@geicp.com

2. Glass Expansion www.geicp.com 1 Limitations of Room Temperature Spray Chambers Intensity drift with temperature Excessive plasma loading (volatile solvents) Excessive oxide formation Insufficient control of analyte transport

3. Glass Expansion www.geicp.com 1 Limitations of Externally Controlled Spray Chambers Requires antifreeze solution Requires bulky floor model chiller Messy coolant lines from chiller to chamber Freezing of condensate on lines Requires intricate jacketed chamber

4. Glass Expansion www.geicp.com 1 IsoMist™ Controlled Temperature Spray Chamber

5. Glass Expansion www.geicp.com 1 IsoMist Encapsulated Spray Chamber

6. Glass Expansion www.geicp.com 1 Features of IsoMist Powerful Peltier-effect chiller reaches -5C in 15 minutes Programmable from -10 to 60C in 1 degree increments Maintains temperature to within 0.1 degree Custom software runs from any PC Compact design (7.5x4x4 inches) 100% self-contained (no external lines) Incorporates Bluetooth® technology for clean wireless control (USB available)

7. Glass Expansion www.geicp.com 1 PC Screen showing IsoMist Software

8. Glass Expansion www.geicp.com 1 Applications of the IsoMist Analyze volatile solvents Reduce oxide interferences Lower detection limits Increase stability

9. Glass Expansion www.geicp.com 1 Determination of Trace Metals in Naphtha Interfere with the cracking process Poisoning of the catalysts Environmental release concerns Origin and migration markers

10. Glass Expansion www.geicp.com 1 Analysis of Naphtha by ICP-OES PE Optima 2100 DV (Axial view) Forward Power: 1500Watts Coolant flow: 20L/min Auxiliary flow: 1.8L/min Nebulizer gas flow: 0.35L/min Injector: 1mm capillary bore Spray Chamber: Twister baffled cyclonic Nebulizer: SeaSpray glass concentric Uptake rate: 300ul/min IsoMist temperature: -10C

11. Glass Expansion www.geicp.com 1 IsoMist on Optima 2100DV

12. Glass Expansion www.geicp.com 1 Spike Recoveries in Naphtha

13. Glass Expansion www.geicp.com 1 Oxide Reduction in ICP-MS Isobaric oxide interferences Element/IsotopeInterference 56 Fe 40 Ar 16 O + 51 V +35 Cl 16 O + 44 Ca +14 N 14 N 16 O + 48 Ti +32 S 16 O + 52 Cr +34 S 18 O + 64 Zn +32 S 16 O 16 O + 64 Zn +48 Ca 16 O + Robert Thomas, Practical Guide to ICP-MS, Marcel Dekker, NY, 2004.

14. Glass Expansion www.geicp.com 1 Reducing Oxides for ICP-MS Data courtesy of David Jones, ALS Chemex, Brisbane, Australia

15. Glass Expansion www.geicp.com 1 Analysis of Limited Sample Volumes Biological applications Intracellular fluids Neonatal samples Forensic applications Requires micro flow nebulization Low DL’s desirable

16. Glass Expansion www.geicp.com 1 Effect of Temperature on Normalized Intensity ( 20ul/min Uptake) ICP-OES PE Optima 2100DV

17. Glass Expansion www.geicp.com 1 Effect of Temperature on DL 20ul/min Uptake (ICP-OES) PE Optima 2100DV

18. Glass Expansion www.geicp.com 1 Stability Enhancement ICP-OES WithoutIsoMist IsoMist At 21C

19. Glass Expansion www.geicp.com 1 IsoMist Compatibility PE ICP & ICP-MS Varian ICP & ICP-MS Agilent ICP-MS ThermoFisher ICP & ICP-MS Horiba J-Y ICP Teledyne Leeman ICP GBC ICP Spectro ICP Borosilicate glass Quartz Inert PFA Models Spray Chamber Materials