1. EPIC-Germany FFQ Jakob Linseisen Nutritional Epidemiology Division of Cancer Epidemiology German Cancer Research Centre, Heidelberg (food frequency questionnaire) Long-term reproducibility of the

2. Page 2 | Nutritional Epidemiology Cancer Epidemiology Follow-up of the EPIC-Heidelberg cohort

3. Page 3 | Nutritional Epidemiology Cancer Epidemiology Information collected during 2 nd follow-up (EPIC-HD) Second FUP: Lifestyle questionnaire Sociodemograhic variablesOccupation, Marital status, Smoking status Physical activity Medical historyCurrent medication incl. hormones, family cancer history, incident diseases Dietary supplementsVitamins, minerals, supplements, naturopathy Food frequency questionnaire Diet and alcohol consumptionFood and nutrient intake, alcoholic beverages, food preparation methods For women Reproductive factorsyear of menopause, oral contraceptives and hormone use, ovarectomy, hysterectomy

4. Page 4 | Nutritional Epidemiology Cancer Epidemiology 2 nd FFQ in EPIC-Heidelberg

5. Page 5 | Nutritional Epidemiology Cancer Epidemiology 2 nd FFQ in EPIC-HD: Questions on food preparation methods and preferred degree of browning

6. Page 6 | Nutritional Epidemiology Cancer Epidemiology EPIC-HD, FFQ2: Participation rate No. participants 1 24.956 Deceased332 Address uncorrect4924.575 No reply for FUP21.413 Refused (permanent)607 Short questionnaire1.017 Lifestyle quest. only76 Questionnaires back 21.462  87.3 % 1 Cohort size: previous cohort – deceased – refused (permanent) – unknown address

7. Page 7 | Nutritional Epidemiology Cancer Epidemiology EPIC-HD, FFQ2: Re-contacting of participants by telephone  5,166 out of 21,462 participants were re-contacted  24 %  Duration of phone calls: roughly 10-15 min (5-30 min)  1,100 hours or 135 working days  Re-contacting if- an entire page was empty; - no answer within an entire food group; - missing answer to “overview”questions; [This overlapped with phone calls for missing data in life-style questionnaires]

8. Page 8 | Nutritional Epidemiology Cancer Epidemiology EPIC-HD, follow-up 2: Re-contacting of participants by telephone Consequence of modification of conditions for re-contacting participants Re-contact because of after 9000 quest.overall missings in … (% praticipants re-contacted) life-style quest.18 %10 % FFQ17 %19 % both questionnaires9 %5 % Total 43%34 % 24 %

9. Page 9 | Nutritional Epidemiology Cancer Epidemiology Characteristics of the study cohort (n=20,733) at baseline (1994-98) and 2nd FUP (2001-03; mean follow-up time of 5.75 years) Men (9530)Women (11203) BaselineFUP2BaselineFUP2 (%) AGE (YEARS) <4018.1-0.0- 40-4934.434.639.017.7 50-5932.432.342.438.8 60-6915.131.518.641.6 70+-1.6-1.9 BODY MASS INDEX (BMI) <18.51.4 0.2 18.5-2554.553.530.830.3 25-3030.031.051.952.4 30-3510.210.114.614.4 >=353.93.72.5 CURRENT EMPLOYMENT Full time27.824.377.055.0 Part time38.132.42.53.8 Retired9.820.316.536.5 Jobless3.22.13.42.6 Not employed (household, parental leave, education) 21.019.20.60.9 (Nagel et al., Br. J. Nutr. 98:194-200, 2007)

10. Page 10 | Nutritional Epidemiology Cancer Epidemiology EPIC-HD, FFQ2: … some results The long-term correlation coefficients ranged from 0.41 (vegetables in men) to 0.77 (alcoholic beverage consumption in women). Consistently for food groups, 60% to 70% of the participants in both genders were re-classified to the same or adjacent quintile of intake (weighted kappa statistics). The median intake of potatoes, added fats, sugar/confectionary, cakes and alcoholic beverages was lower in the second than in the first FFQ, whereas the median intake of fruits, vegetables, cereals/cereal products and non-alcoholic beverages were higher. (Nagel et al., Br. J. Nutr. 98:194-200, 2007)