1. What is Life Lab??

2. DAY 1

3. Mystery Substancce 1Mystery Substancce 2

4. Write Objective and Day 1 Materials

5. Mystery 2 Mystery 1 Picture Set up Notebook…… Observations: 1. 2. 3. Observations: 1. 2. 3. Predict: living or dead or nonliving Predict: living or dead or nonliving Life-like charact. Non life-like charact. Life-like charact. Non life-like charact. ID substance ID substance

6. Examine mystery objects using magnify glass &/or the stereo microscope – make a scientific drawing in your log booksstereo microscope scientific drawing Highly detailed Accurate Appropriately scaled Clearly labeled Before we begin……

7. Examples of Student Scientific Drawings

9. Before we begin…… Living, Dead, or Non-living? Something that is alive.. Satisfies ALL 8 characteristics of life Is not alive, and has never lived Was once alive

10. Begin observations

11. Mystery Substance 1 Living, Dead, or Non-living? Living - Potential for life Green food coloring in water

12. Living, Dead, or Non-living? Living - Potential for life Brine Shrimp Eggs Mystery Substance 2

13. It’s not always easy to ID life. Some dormant, or slow-growing organisms may appear to be nonliving unless observed under the right conditions or over a period of time.

15. Problem/Question  How does salinity affect the number of brine shrimp eggs that will hatch?  Independent Variable: –Concentration of Salt  Dependent Variable –Number of living brine shrimp Write Your Hypothesis

16. If the ________ ___________ then the ________will ________________ IVHow IV changes DV What you think DV will do

17. Materials brine shrimp eggs 0%, 1%, 3%, 6% saline solution (sea salt) Sealed plastic Test tubes stereo microscope stirring rod toothpick well slide and cover slip metric ruler

18. Procedures 1) put 20mL of each solution into a test tube and label with tape. 2-0 2-12-32-6 Group # and % on each test tube If you are group 2: Ex) [2-0] [2-1] [2-3] [2-6]

19. 2) Mark a toothpick at 1.5 cm 3) wet toothpick up to mark

20. 4) Dip into brine shrimp eggs 5) Put toothpick into 0% solution test tube and stir until the eggs have come off 2-0 2-12-32-6

21. 5) REPEAT STEPS 2-5 WITH 1%, 3%, AND 6% SALINE SOLN 6) CAP TEST TUBES

22. DAY 2

23. 1) Suck up sample of 0%/shrimp mix into pipette 2-0 2-12-32-6 2) Put 10 drops into well slide

24. 3) Examine under dissecting microscope 4) illustrate, comment Data Table 1

26. 5) Count # hatched and unhatched in your sample 6) Calculate total # hatched and unhatched by multiplying sample # by 20 (b/c there are 20 ml in test tube.. And you examined 1 ml) 7) Suck up the solution from the well 2-0 2-12-32-6 8) Return to appropriate test tube

27. 9) REPEAT STEPS 1-8 WITH 1%, 3%, AND 6% SALINE SOLN

28. DAY 3 Same as Day 2

29. Analysis and Conclusion  Combine class data and take class average for Days 1 – 3  Construct a graph using the class averages  Write conclusion