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Chapter 16 DNA Replication. Base Pairing A forms 2 H bonds with T G forms 3 H bonds with G In RNA, A forms 2 H bonds with U Complimentary strands Anti-parallel.

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Presentation on theme: "Chapter 16 DNA Replication. Base Pairing A forms 2 H bonds with T G forms 3 H bonds with G In RNA, A forms 2 H bonds with U Complimentary strands Anti-parallel."— Presentation transcript:

1 Chapter 16 DNA Replication

2 Base Pairing A forms 2 H bonds with T G forms 3 H bonds with G In RNA, A forms 2 H bonds with U Complimentary strands Anti-parallel orientation

3 Proteins Involved in DNA Replication Helicase Topoisomerase Single stranded binding proteins Primase DNA Polymerase III DNA Polymerase I DNA Ligase

4 Steps in DNA Replication Open the strands to access the nitrogenous bases Prime Build leading strand Build lagging strand Repair Open next segment

5 Opening the DNA Origins of replication Helicase Replication fork Replication bubble Antiparallel alignment Single stranded binding proteins Topoisomerases

6 Formation of Replication Fork

7 Priming DNA Replication Primase RNA primer Free 3’ hydroxyl 5’-3’ progression of newly manufactured strand Template strand in 3’- 5’ direction

8 Building DNA DNA Polymerase III –5’-3’ new strand –3’-5’ template strand Leading strand –Toward replication fork –continuous Lagging strand –Away from replication fork –discontinuous –Okazaki fragments

9 Clean up DNA Polymerase I –Removes RNA primer –Fills in gap DNA Ligase –Closes nicked region

10 Summary

11 Movies and Videos http://www.youtube.com/watch?v=teV62zrm2P0 &feature=relatedhttp://www.youtube.com/watch?v=teV62zrm2P0 &feature=related http://www.wiley.com/college/pratt/0471393878/ student/animations/dna_replication/index.htmlhttp://www.wiley.com/college/pratt/0471393878/ student/animations/dna_replication/index.html http://www.bioteach.ubc.ca/TeachingResources/ MolecularBiology/DNAReplication.swfhttp://www.bioteach.ubc.ca/TeachingResources/ MolecularBiology/DNAReplication.swf http://bcs.whfreeman.com/thelifewire/content/ch p11/1102002.htmlhttp://bcs.whfreeman.com/thelifewire/content/ch p11/1102002.html http://www.youtube.com/watch?v=4jtmOZaIvS0 http://www.youtube.com/watch?v=dIZpb93NYlw

12 Replicating the Ends of DNA Molecules Chromosome ends Repetitive sequences Shorten with each replicative cycle Fray Time keeper 50 cycles

13 DNA Organization Histone proteins Nucleosomes Chromosomes Condensed only for cell division

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