1. Genomics: READING genome sequences ASSEMBLY of the sequence ANNOTATION of the sequence carry out dideoxy sequencing connect seqs. to make whole chromosomes find the genes! “FIRST” generation sequencing

2. M.L. Metzker 2010. Nature Genetics Reviews 11, 31. First, 5 slides, in order of Figs. Ion torrent Nanopore

3. 5 Selected Technologies. 2 nd, 3 rd, and 4 th generation sequencing. Using Metzker, M.L. 2010, and T.P. Niedringhaus 2011Anal. Chem. 83, 4327. Solexa - - ->Illumina

7. Allows both directions: “pair-end”

9. Life: Roche/454; Polonator Soon to be bought by Thermo Fischer

12. NONE USED IN ROCHE/LIFE 454 PYROSEQUENCING

13. Ion Torrent (BIU) http://www.iontorrent.com/1-trillion-front-end/

18. Pacific Biosciences

21. New Generation: Template With hairpins On each end

23. Nanopore DNA sequencing

24. http://lifetech-it.hosted.jivesoftware.com/videos/1016 ??? work

28. Illumina Solexa 2015 HiSeq 3000 and HiSeq 4000 are Illumina’s next iteration sequencing system for the current HiSeq 2500. HiSeq 3000 has single flow cell, while HiSeq 4000 has two flow cells. Illumina brings its patterened flow cell design to the HiSeq 3000 and HiSeq 4000 and makes the to deliver more data at lower cost than the current HiSeq 2500. Illumina claims that the HiSeq 4000 can sequence up to 12 genomes, 100 whole transcriptome samples, or 180 exomes in 3.5 days or less.