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Analysis of amino acids and proteins

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Presentation on theme: "Analysis of amino acids and proteins"— Presentation transcript:

1 Analysis of amino acids and proteins
Amino acids- ninhydrin and TLC Proteins- biuret test and electrophoresis

2 Mixture of aminoacids Separate by thin layer chromatography (TLC)
Detect with ninhydrine (spray and heat) LILAC!

3 Mixture of proteins or aminiacids
Separate with chromatography- electrophoresis Separation by molecular weight and charge PAGE- PolyAcrylamide Gel Electrophoresis

4 PAGE

5 Detection of proteins Detect with biuret test- add Cu2+ and NaOH, lilac coulour indicates protein. Complexes with the peptide bonds and forms a violet complex

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