1. DNA REPLICATION

2. MAIN IDEAS  What exactly happens in the S phase? How does this occur?  What does 3’ and 5’ stand for?  Looking at the website, does replication go in one- way direction? If yes, which direction?  What does semi- conservative mean?  What is the difference between the leading strand and lagging strand?  At the end of replication, how do the strands compare with each other?  USING YOUR NOTES, YOUR MEMORY AND THE WEBSITE BELOW, TRY AND ANSWER THE ABOVE QUESTIONS: https://lpscience.fatcow.com/jwanamaker/animations/DNA%20Re plication%20-%20long%20.html https://lpscience.fatcow.com/jwanamaker/animations/DNA%20Re plication%20-%20long%20.html

4. THE 5 W’s of Replication:  What are the 5W’s in DNA REPLICATION:  WHO is involved (are the major protein players)?  WHAT is being accomplished:  WHEN does it occur in the cell cycle:  WHERE does it occur in prokaryotes vs eukaryotes?  EUKARYOTIC CELLS- PROKARYOTIC CELLS-  WHY does replication happen:

5. THE 5W’s in DNA REPLICATION:  WHO is involved (are the major protein players): Helicase, SSB, DNA Polymerase, Primase, ligase  WHAT is being accomplished: DNA REPLICATION  WHEN does it occur in the cell cycle: S PHASE  WHERE does it occur in prokaryotes vs eukaryotes?  EUKARYOTIC CELLS- NUCLEUSPROKARYOTIC CELLS- cytoplasm  WHY does replication happen: TO MAKE MORE DNA (FOR MITOSIS)

6. HOW does DNA REPLICATION occur????  http://www.bioteach.ubc.ca/TeachingResources/MolecularBiology /DNAReplication.swf http://www.bioteach.ubc.ca/TeachingResources/MolecularBiology /DNAReplication.swf  http://www.stolaf.edu/people/giannini/flashanimat/molgenetics/dn a-rna2.swf http://www.stolaf.edu/people/giannini/flashanimat/molgenetics/dn a-rna2.swf

7. MAJOR PLAYERS: WHAT DO THEY DO??? Helicase SSB DNA polymerase RNA primase Ligase  NOTES: FINISH DNA notes and collect (overview)//  ACT OUT…  VISUALIZE: AMEOBA SISTERS REPLICATION: https://www.youtube.com/watch?v=5qSrmeiWsuc https://www.youtube.com/watch?v=5qSrmeiWsuc

8.  DNA REPLICATION PAPER CLIP//PIPE CLEANER ACTIVITY  MUTATIONS …