1. About OMICS Group OMICS Group International is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of making the information on Sciences and technology ‘Open Access’, OMICS Group publishes 400 online open access scholarly journals in all aspects of Science, Engineering, Management and Technology journals. OMICS Group has been instrumental in taking the knowledge on Science & technology to the doorsteps of ordinary men and women. Research Scholars, Students, Libraries, Educational Institutions, Research centers and the industry are main stakeholders that benefitted greatly from this knowledge dissemination. OMICS Group also organizes 300 International conferences annually across the globe, where knowledge transfer takes place through debates, round table discussions, poster presentations, workshops, symposia and exhibitions. Open Access publicationsscholarly journalsInternational conferencesOpen Access publicationsscholarly journalsInternational conferences

2. About OMICS Group Conferences OMICS Group International is a pioneer and leading science event organizer, which publishes around 400 open access journals and conducts over 300 Medical, Clinical, Engineering, Life Sciences, Phrama scientific conferences all over the globe annually with the support of more than 1000 scientific associations and 30,000 editorial board members and 3.5 million followers to its credit. OMICS Group International is a pioneer and leading science event organizer, which publishes around 400 open access journals and conducts over 300 Medical, Clinical, Engineering, Life Sciences, Phrama scientific conferences all over the globe annually with the support of more than 1000 scientific associations and 30,000 editorial board members and 3.5 million followers to its credit. OMICS Group has organized 500 conferences, workshops and national symposiums across the major cities including San Francisco, Las Vegas, San Antonio, Omaha, Orlando, Raleigh, Santa Clara, Chicago, Philadelphia, Baltimore, United Kingdom, Valencia, Dubai, Beijing, Hyderabad, Bengaluru and Mumbai. OMICS Group has organized 500 conferences, workshops and national symposiums across the major cities including San Francisco, Las Vegas, San Antonio, Omaha, Orlando, Raleigh, Santa Clara, Chicago, Philadelphia, Baltimore, United Kingdom, Valencia, Dubai, Beijing, Hyderabad, Bengaluru and Mumbai.

3. Therapeutic Potentials of Plant Extracts with reference to Anti-obesity Therapeutic Potentials of Plant Extracts with reference to Anti-obesity Date: 26/08/2014 2nd International Conference on Pharmacognosy, Phytochemistry and Natural Products, 25-27 August 2014, Beijing, China 3 Name of the Speaker: Mandavi Deshpande-Garge Research Center: APT Research Foundation, S. No.36/1/1,M.N.199, Vadgaon Khurd, Sinhagad Road, Pune 411 041, Maharashtra, India.

4. Introduction Nature a Great Chemist Ancient Science of Ayurveda & Unani Around 120 drugs originated from Plants Chemically synthesized drugs Standardization of chemically synthesized drugs Efficacy studies of these drugs Date: 26/08/2014 2nd International Conference on Pharmacognosy, Phytochemistry and Natural Products, 25-27 August 2014, Beijing, China 4

5. Use of Plant Originated Drugs Use of Plant Originated Drugs Disease condition Name of the drugName of the plant MalariaQuinineCinchona ledgeriana DysenteryAesculetin Neoandrographolide, Andrographolide Frazinus rhychophylla Andrographis paniculata CancerTaxol Betulinic acid Taxus brevifolia Betula alba, Ziziphus mauritiana CardiovascularDigitalin,DigitoxinDigitalis purpurea Kidney disfunctionTheophyllineTheobroma cacao Liver DamageSilymarinSilybum marianum Parkinson’sL-DopaMucuna spp CNSCaffeineCamellia sinensis 5 Ref: Plant-Based Drugs and Medicines by Leslie Taylor Date: 26/08/2014 2nd International Conference on Pharmacognosy, Phytochemistry and Natural Products, 25-27 August 2014, Beijing, China

6. Use of Plant Extracts contd.. Use of Plant Extracts contd.. Disease conditionName of the PlantPart of the plant in use DiabetesTrigonella foenum- graecum Momordica charantia Seeds Fruit ObesityGarcinia cambogia Commiphora mukul Fruit Stem CardiovascularTerminalia arjunaBark Liver DamageSilybum marianum Curcuma longa Camellia sinensis Leaves Rhizome Leaves 6 Date: 26/08/2014 2nd International Conference on Pharmacognosy, Phytochemistry and Natural Products, 25-27 August 2014, Beijing, China

7. Diseases Associated with Obesity Diabetes (Ref.: NIH,USA) Cardiovascular Diseases (Ref.: NIH,USA) Cancer of Oesophagus, Pancreas, Colon and Rectum, Breast (after menopause), Endometrium (lining of the uterus), Kidney, Thyroid, Gallbladder, (Ref.: NCI, NIH,USA) Infertility (Ref.: NIH,USA) Inflammation (Ref.: NIH,USA) 7 Date: 26/08/2014 2nd International Conference on Pharmacognosy, Phytochemistry and Natural Products, 25-27 August 2014, Beijing, China

8. Lipid Metabolism 8 Source: Part Three: Ray medina, Dietary Fat, Chylomicrons and Endotoxemia Published November 26, 2012. Date: 26/08/2014 2nd International Conference on Pharmacognosy, Phytochemistry and Natural Products, 25-27 August 2014, Beijing, China

9. Probable Mechanisms for Anti-Obesity Activity ↓ Lipid absorption ↓ Energy intake ↑ Energy expenditure ↓ Pre-adipocyte differentiation and proliferation ↓ Lipogenesis and ↑ lipolysis Ref: Jong Won Yun, Possible anti-obesity therapeutics from nature – A review Phytochemistry 71 (2010) 1625–1641. 9 Date: 26/08/2014 2nd International Conference on Pharmacognosy, Phytochemistry and Natural Products, 25-27 August 2014, Beijing, China

10. Plant material Ziziphus mauritiana (Ber) Authentication certificate 10 Date: 26/08/2014 2nd International Conference on Pharmacognosy, Phytochemistry and Natural Products, 25-27 August 2014, Beijing, China

11. Quantitative Phytochemical Analysis of Ziziphus mauritiana bark powder (ZMBP) extracts. 11 Extract Phytochemical constituents Tannin as Catechin Flavonoid as Quercetin Phenol as Gallic acid ChloroformAbsent Ethanol120 mg/gm101.2 mg/gmNegligible Aqueous165 mg/gm105 mg/gmNegligible Tannins : Adipocyte differentiation inhibitor Flavonoids : Increase Glucose uptake Date: 26/08/2014 2nd International Conference on Pharmacognosy, Phytochemistry and Natural Products, 25-27 August 2014, Beijing, China

12. Anti-Oxidant Activity 12 ExtractConcentration µg/ml % Inhibition Concentration Equivalent to ascorbic acid µg/ml Chloroform10040.8112.59 Ethanol100 39.27 Aqueous100 39.27 % Inhibition of ABTS + oxidation by ZMBP Extracts Date: 26/08/2014 2nd International Conference on Pharmacognosy, Phytochemistry and Natural Products, 25-27 August 2014, Beijing, China

13. Lipase Inhibitory Activity 13 % inhibition of Porcine Pancreatic Lipase activity by ZMBP Aqueous Extract Concentration of Aqueous Extract µg/ml Inhibition of Lipase 10040% 20072% 400100% Date: 26/08/2014 2nd International Conference on Pharmacognosy, Phytochemistry and Natural Products, 25-27 August 2014, Beijing, China

14. In Vitro Studies Cell Viability Assay in L929 cell line 14 Date: 26/08/2014 2nd International Conference on Pharmacognosy, Phytochemistry and Natural Products, 25-27 August 2014, Beijing, China

15. In Vitro Studies 15 3T3-L1 Adipocyte Differentiation assay (Magnification 40X) Date: 26/08/2014 2nd International Conference on Pharmacognosy, Phytochemistry and Natural Products, 25-27 August 2014, Beijing, China

16. 2nd International Conference on Pharmacognosy, Phytochemistry and Natural Products, 25-27 August 2014, Beijing, China16 50 nM Insulin stimulationVehicle Control C-10 micro g/ml C-50 micro g/ml Glut 4 Translocation Assay in CHO-HIRC-muc-GLUT4eGFP cells

17. Glucose Uptake Assay in CHO-HIRC-muc-GLUT4eGFP cells 17 Date: 26/08/2014 2nd International Conference on Pharmacognosy, Phytochemistry and Natural Products, 25-27 August 2014, Beijing, China z

18. In Vivo Studies Development of Obese B6D2F1 mice High Fat Diet for 30 days Dosing the animals (n=6) for 30 days at 100 mg/kg body weight, orally with ZMBP Aqueous extract (ZMBPE aq.) & Standard drug Orlistat 7.8 mg/kg Monitoring of Obesity parameters Body weight, Anthropometry, Insulin resistance on day 0 and day 30 Weight of Peritoneal and Epididymal Fat Pad Tissue Triglycerides Determination Tissue Triglycerides Determination Pancreatic Lipase activity 18 Date: 26/08/2014 2nd International Conference on Pharmacognosy, Phytochemistry and Natural Products, 25-27 August 2014, Beijing, China

19. In Vivo Studies Anti-Obesity Activity in Mice: Body Weight Data 19 GroupDay 7Day 14Day 21Day 30 ZMBPE aq.2.6412.3520.32***20.71*** Std10.03*15.5821.99***27.39*** % reduction in Body weight gain *= P<0.05, ***=P<0.001 Date: 26/08/2014 2nd International Conference on Pharmacognosy, Phytochemistry and Natural Products, 25-27 August 2014, Beijing, China

20. In Vivo Studies Effect of ZMBPE aq. on Diet Induced Obese Mice Body Weight Data and Anthropometrical Parameters In Vivo Studies Effect of ZMBPE aq. on Diet Induced Obese Mice Body Weight Data and Anthropometrical Parameters 20 **=p<0.01, ***=p<0.001 GroupBody weight in grams Thoracic Circumference in cms Abdominal Circumference in cms Epididymal Fat tissue weight in grams Peritoneal Fat in grams Normal Control 30.98 ± 0.886.8 ± 0.247.3 ± 0.250.18 ± 0.050.25 ± 0.08 Obese Control 34.42 ± 2.627.4± 0.168.1 ± 0.520.45 ± 0.101.20 ± 0.48 ZMBPE aq. 28.08 ± 1.28 *** 6.6 ± 0.47***6.3 ± 0.32 ** 0.12 ± 0.030.49 ± 0.14 ** Standard 25.72 ± 2.21 *** 5.8 ± 0.40 *** 5.9 ± 0.44 *** 0.07 ± 0.020.13 ± 0.06 ** Date: 26/08/2014 2nd International Conference on Pharmacognosy, Phytochemistry and Natural Products, 25-27 August 2014, Beijing, China

21. In Vivo Studies Effect of ZMBPE aq. on Diet Induced Obese Mice 21 Normal ControlObese ControlZMBPE aq. Date: 26/08/2014 2nd International Conference on Pharmacognosy, Phytochemistry and Natural Products, 25-27 August 2014, Beijing, China

22. In Vivo Studies Effect of ZMBPE aq. on Diet Induced Obese Mice : Lipid Profile, Tissue triglycerides and Faecal Fat/ Pancreatic Lipase Analysis 22 Date: 26/08/2014 2nd International Conference on Pharmacognosy, Phytochemistry and Natural Products, 25-27 August 2014, Beijing, China Lipid Profile Tissue Triglyceride content Faecal fat and pancreatic lipase analysis

23. Summary o All extracts showed Anti-oxidant activity o The ZMBPE Aq. showed Lipase inhibitory activity o ZMBPE Aq inhibited the pre-adipocytes differentiation induced by IBMX + insulin. o It also showed Insulin-like glucose transport stimulatory activity in CHO-HIRC-muc-GLUT4eGFP cells. o In the present studies we have observed reduction in body weight gain, decrease in triglyceride content, decrease in pancreatic lipase activity associated with increased faecal lipid excretion in ZMBPE Aq. fed obese mice. 23 Date: 26/08/2014 2nd International Conference on Pharmacognosy, Phytochemistry and Natural Products, 25-27 August 2014, Beijing, China

24. Acknowledgement Dr. P. B. Parab and Dr. K. G. Apte, APT Research Foundation Pune, Maharashtra, India. Dr. Vijayakumar and Dr Manoj Kumar Bhat, of National Centre For Cell Sciences, Pune, Maharashtra, India. All Staff, National Toxicology Centre and APT Research Foundation, Pune, Maharashtra, India. 24 Date: 26/08/2014 2nd International Conference on Pharmacognosy, Phytochemistry and Natural Products, 25-27 August 2014, Beijing, China

25. 25 Date: 26/08/2014 2nd International Conference on Pharmacognosy, Phytochemistry and Natural Products, 25-27 August 2014, Beijing, China THANK YOU

26. Lets Meet again at Pharmacognosy-2015 3 rd International Conference and Exhibition on Pharmacognosy, Phytochemistry and Natural Products October 26-28, 2015 Hyderabad, India Theme: Advanced trends for the future of Herbal Drugs and Products Website: http://pharmacognosy-phytochemistry-natural- products.pharmaceuticalconferences.com/ http://pharmacognosy-phytochemistry-natural- products.pharmaceuticalconferences.com/http://pharmacognosy-phytochemistry-natural- products.pharmaceuticalconferences.com/