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John B. Cole Animal Genomics and Improvement Laboratory Agricultural Research Service, USDA Beltsville, MD 20705-2350 2015 Using.

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Presentation on theme: "John B. Cole Animal Genomics and Improvement Laboratory Agricultural Research Service, USDA Beltsville, MD 20705-2350 2015 Using."— Presentation transcript:

1 John B. Cole Animal Genomics and Improvement Laboratory Agricultural Research Service, USDA Beltsville, MD 20705-2350 john.cole@ars.usda.gov 2015 Using genotypes to construct phenotypes for dairy cattle breeding programs and beyond

2 Final OptiMIR Scientific and Expert Meeting, Namur, Belgium, April 17, 2015 (‹#›) Cole Why do we need new phenotypes? ● Changes in production economics ● Technology enables collection of new phenotypes ● Better understanding of biology ● Recent review by Egger-Danner et al. (2015) in Animal

3 Final OptiMIR Scientific and Expert Meeting, Namur, Belgium, April 17, 2015 (‹#›) Cole Source: Miglior et al. (2012) Selection indices now include many traits

4 Final OptiMIR Scientific and Expert Meeting, Namur, Belgium, April 17, 2015 (‹#›) Cole New phenotypes should add information lowhigh Genetic correlation with existing traits low high Phenotypic correlation with existing traits Novel phenotypes include some new information Novel phenotypes include much new information Novel phenotypes contain some new information Novel phenotypes contain little new information

5 Final OptiMIR Scientific and Expert Meeting, Namur, Belgium, April 17, 2015 (‹#›) Cole Cost of measurement vs. value to farmers low high Cost of measurement low high Value of phenotype (milk yield) (greenhouse gas emissions) (feed intake) (conformation)

6 Final OptiMIR Scientific and Expert Meeting, Namur, Belgium, April 17, 2015 (‹#›) Cole Some novel phenotypes studied recently ● Claw health (Van der Linde et al., 2010) ● Dairy cattle health (Parker Gaddis et al., 2013) ● Embryonic development (Cochran et al., 2013) ● Immune response (Thompson-Crispi et al., 2013) ● Methane production (de Haas et al., 2011) ● Milk fatty acid composition (Soyeurt et al., 2011) ● Persistency of lactation (Cole et al., 2009) ● Rectal temperature (Dikmen et al., 2013) ● Residual feed intake (Connor et al., 2013)

7 Final OptiMIR Scientific and Expert Meeting, Namur, Belgium, April 17, 2015 (‹#›) Cole What do US dairy farmers want? ● National workshop in Tempe, AZ in February ● Producers, industry, academia, and government ● Farmers want new tools ● Additional traits (novel phenotypes) ● Better management tools ● Foot health and feed efficiency were of greatest interest

8 Final OptiMIR Scientific and Expert Meeting, Namur, Belgium, April 17, 2015 (‹#›) Cole What can farmers do with novel traits? ● Put them into a selection index ● Correlated traits are helpful ● Apply selection for a long time ● There are no shortcuts ● Collect phenotypes on many daughters ● Repeated records of limited value ● Genomics can increase accuracy

9 Final OptiMIR Scientific and Expert Meeting, Namur, Belgium, April 17, 2015 (‹#›) Cole TraitBias* Reliability (%) Reliability gain (% points) Milk (kg)−80.369.230.3 Fat (kg)−1.468.429.5 Protein (kg)−0.960.922.6 Fat (%)0.093.754.8 Protein (%)0.086.348.0 Productive life (mo)−0.773.741.6 Somatic cell score 0.064.929.3 Daughter pregnancy rate (%) 0.253.520.9 Sire calving ease 0.645.819.6 Daughter calving ease−1.844.222.4 Sire stillbirth rate 0.228.2 5.9 Daughter stillbirth rate0.137.617.9 Holstein prediction accuracy *2013 deregressed value – 2009 genomic evaluation

10 Final OptiMIR Scientific and Expert Meeting, Namur, Belgium, April 17, 2015 (‹#›) Cole Constructing phenotypes from genotypes ● Prediction from correlated traits or phenotypes from reference herds ● Haplotypes can be used when causal variants are not known ● Causal variants can be used in place of markers ● Specific combining abilities can combine additive and dominance effects

11 Final OptiMIR Scientific and Expert Meeting, Namur, Belgium, April 17, 2015 (‹#›) Cole Genotypes are abundant

12 Final OptiMIR Scientific and Expert Meeting, Namur, Belgium, April 17, 2015 (‹#›) Cole Example: Polled cattle ● Polled cattle have improved welfare and increased economic value ● polled haplotypes have low frequencies: 0.41% in BS, 0.93% in HO, and 2.22% in JE ● Increasing haplotype frequency by index selection requires known status for all animals ● Estimate gene content (GC) for all non- genotyped animals.

13 Final OptiMIR Scientific and Expert Meeting, Namur, Belgium, April 17, 2015 (‹#›) Cole Prediction of gene content ● The densefreq.f90 program (VanRaden) was modified to use the methodology of Gengler et al. (2007) ● Information from all genotyped relatives used ● Gene content is real-valued and continuous in the interval [0,2].

14 Final OptiMIR Scientific and Expert Meeting, Namur, Belgium, April 17, 2015 (‹#›) Cole Addition of polled to the Net Merit index ● $11.79 (€10.85) and $10.73 (€9.87) for costs of dehorning and polled genetics, respectively (Widmar et al., 2013) ● Haplotype count multiplied by $22.52 (€20.72) for genotyped animals ● Gene content multiplied by $22.52 (€20.72) for non-genotyped animals ● Rank correlations with 2014 NM$ compared for bulls and cows

15 Final OptiMIR Scientific and Expert Meeting, Namur, Belgium, April 17, 2015 (‹#›) Cole Validation of Jersey polled gene content ● Polled status from recessive codes and animal names compared to GC for 1,615 non-genotyped JE with known status. ● 97% (n = 675) of pp animals correctly assigned GC near 0 ● Pp animals had GC near 0 (52%, n = 474) and near 1 (47%; n = 433) ● All PP animals (n = 11) assigned GC of ~2.

16 Final OptiMIR Scientific and Expert Meeting, Namur, Belgium, April 17, 2015 (‹#›) Cole Reasons for variation in gene content ● The expectation for GC is near 1 for heterozygotes ● GC can be <1 if many polled ancestors have unknown status or when pedigree is unknown ● In those cases GC may be set to twice the allele frequency, which is low for polled ● Some animals with -P in the name may actually be PP, not Pp

17 Final OptiMIR Scientific and Expert Meeting, Namur, Belgium, April 17, 2015 (‹#›) Cole Gene content for polled in Jerseys MAF = 2.5% pp PpPP

18 Final OptiMIR Scientific and Expert Meeting, Namur, Belgium, April 17, 2015 (‹#›) Cole Jersey polled merit GroupNρ All animals2,471,0250.99997 All cows2,436,4390.99997 All bulls34,5860.99990 Young bulls (G status)3800.99787

19 Final OptiMIR Scientific and Expert Meeting, Namur, Belgium, April 17, 2015 (‹#›) Cole Validation of Holstein polled gene content ● Polled status from recessive codes and animal names compared to GC for 1,615 non-genotyped JE with known status. ● 97% (n = 675) of pp animals correctly assigned GC near 0 ● Pp animals had GC near 0 (52%, n = 474) and near 1 (47%; n = 433) ● All PP animals (n = 11) assigned GC of ~2.

20 Final OptiMIR Scientific and Expert Meeting, Namur, Belgium, April 17, 2015 (‹#›) Cole Allele content for polled in Holsteins MAF = 1.07% pp PpPP

21 Final OptiMIR Scientific and Expert Meeting, Namur, Belgium, April 17, 2015 (‹#›) Cole Holstein polled merit GroupNρ All animals29,010,4570.99999 All cows28,769,8030.99999 All bulls240,6540.99994 Young bulls (G status) 1,6070.99966

22 Final OptiMIR Scientific and Expert Meeting, Namur, Belgium, April 17, 2015 (‹#›) Cole Allele content for DGAT1 in Jerseys MAF = 47.9%

23 Final OptiMIR Scientific and Expert Meeting, Namur, Belgium, April 17, 2015 (‹#›) Cole NameChromeLocation (Mbp)Carrier FreqEarliest Known Ancestor HH1562-684.5Pawnee Farm Arlinda Chief HH2193-984.6Willowholme Mark Anthony HH3892-974.7Glendell Arlinda Chief, Gray View Skyliner HH411.2-1.30.37Besne Buck HH5992-942.22Thornlea Texal Supreme JH11511-1623.4Observer Chocolate Soldier BH1742-4714.0West Lawn Stretch Improver BH21910-127.78Rancho Rustic My Design AH11765.9-66.226.1Selwood Betty’s Commander Other phenotypes may come from genotypes For a complete list, see: http://aipl.arsusda.gov/reference/recessive_haplotypes_ARR-G3.html.

24 Final OptiMIR Scientific and Expert Meeting, Namur, Belgium, April 17, 2015 (‹#›) Cole Conclusions New technology is enabling the collection of novel phenotypes Genotypes are now routinely available for young animals High-density SNP genotypes can be used to construct phenotypes directly

25 Final OptiMIR Scientific and Expert Meeting, Namur, Belgium, April 17, 2015 (‹#›) Cole Acknowledgments Dan Null and Paul VanRaden, AGIL Chuanyu Sun, Sexing Technologies AFRI grant 1245-31000-101-05, “Improving Fertility of Dairy Cattle Using Translational Genomics”

26 Final OptiMIR Scientific and Expert Meeting, Namur, Belgium, April 17, 2015 (‹#›) Cole Questions? http://gigaom.com/2012/05/31/t-mobile-pits-its-math-against-verizons-the-loser-common-sense/shutterstock_76826245/

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