1. ELUTION DIAGRAM OF CYTOCHROME-C FROM ION-EXCHANGE MAGNETIC NANOPARTICLES

2. FERMENTATION PROFILE OF Pichia pastoris:
PRODUCTION OF RECOMBINANT DROSOMYCIN

3. ADSORPTION ISOTHERM OF DROSOMYCIN FERMENTATION BROTH
ON MAGNETIC NANOPARTICLES

4. SUMMARY OF DROSOMYCIN PURIFICATION USING MAGNETIC NANOPARTICLES

5. SDS GEL ELECTROPHORESIS USING MAGNETIC NANOPARATICLES: ELUTION PROFILES OF DROSOMYCIN

6. COMPARISON OF MAGNETIC NANOPARTICLES WITH OTHER PURIFICATION SCHEMES

7. NEW CONCEPT IN INCREASING OXYGEN
TRANSFER RATE USING MAGNETIC NANOPARTICLES
Magnetic
particle
Oleic Acid
20 nm
30-50 nm
Low amount of coating
Very high interfacial areas
Readily recovered by magnetic filtration

8. SYNTHESIS OF MAGNETIC NANO-PARTICLES
Aqueous solution of FeCl2 and FeCl3
NH4OH
80ºC
Oleic acid
coating
Hitenol
Fast (30 min) simple synthesis (stirred tank)
Inexpensive, readily available materials

9. MASS TRANSFER CHARACTERIZATION IN BIOREACTORS: SULFITE OXIDATION
air to mass spec
Di = 10cm
HL = 14.5cm
[SO32-] = 0.67M
[Cu2+] = 1x10-3 M
VTOTAL = 20L
VWORKING = 5.5L
Dtank = 22cm

10. OXYGEN MASS TRANSFER COEFFICIENT VERSUS
GASSED POWER PER UNIT VOLUME IN 20-LITER BIOREACTOR
Ф: particle wt%

11. OXYGEN MASS TRANSFER IN E. coli FERMENTATIONS
Seed culture
- 100 ml LB in 500 ml shake flask
- overnight culture at 37 oC, 220rpm
Fermentation culture (7.5 L fermentor)
- inoculation volume: 10% (v/v)
- initial fermentation volume: 3 L
- temperature: 37 oC
- agitation speed: 600 rpm
- pH = , adjusted by 4 M (NH4OH:NaOH = 2:2)

12. SUMMARY OF OXYGEN TRANSFER COEFFICIENT IN E. COLI FERMEMENTATION WITH
AND WITHOUT MAGNETIC NANOPARTICLES
Particles
(w/v)
Air-flow rate
(L/min)
Oxygen Transfer
(from 7 to 10 hrs)
[mmol O2/(L-h)]
kLa
[mmol O2/(L-h-Atm O2]
Normalized kLa
(air-flow rate = 2 L/min)
Fermentation III
none
2.0
26.68
127.0
Fermentation I
3.0
36.30
172.9
131.8
Fermentation II 2%
1.0
74.04
352.6
561.0
Fermentation IV 4%
122.84
585.0

13. SUMMARY AND CONCLUSIONS
Micro-Bioreactors Will Began to Have Impacts in Biotechnology Processes
Reducing Time in Process Development
Strain Selection
Medium Development
Product Quality in Mammalian Cell Culture
Nano-Technology Has Definite Future
Product Purification
Over-Coming Transport Barriers
Oxygen Transfer
Biocatalysis and Co-Factor Regeneration