1. Determine new application spaces in the sequencing platform using visions systems Jessica Perez Summer Research Program in Genomics Massachusetts Institute of Technology Summer 2007

2. Outline Approach and Goals Vision Systems Potential Application Spaces Demonstrated Application Spaces

3. Project Overview Approach: use Cognex Smart Sensor and Intellect 1.4.0 software as a model to demonstrate the capabilities of vision systems Goals:  Explore capabilities of software  Look for application spaces  Take application and prove principal

4. Vision Systems Software Capabilities Lighting Post Processing

5. Software Capabilities Blob Identification 1D Bar Scanning Flexible Flaw Detection

6. User Interface Result Table Tool Box Viewing Screen

7. Blob Identification Darker Objects Meet Parameters Lighter Objects FAILED IDENTIFICATION CRITERIA Area Average Intensity

8. 1D Code Reader Numerical Code Read PASS

9. Flaw Detection Model PARAMETERS Blob Color Area Perimeter

10. Additional Tests Counting Filtering Measurement 2D String Reader Optical Character Recognition Reader TOOLS

11. Lighting Answer.com FilteringIntensity Geometry

12. Common Lighting Techniques Bright Field Dark Field Back Lighting From Vision Light Tech

13. Post Processing MatLab Histogram Filter BeforeAfter

14. Potential Application Spaces Process: Solexa Screening Consumer: Glass Tubes

15. Process: Solexa Screening Quality Control Check in the Solexa Sequencing Process Clusters Flowcells

16. Proof of Principle Blobs represent clusters of DNA

17. Consumer : Test Tube DNA Sonic Waves Probe Sonication

18. Backlighting BEFORE AFTER Detection of Dimensions and Shape of Transparent Objects Images by Keyence-Engineering Knowledge

19. Setup Components LED Strobe Light Magnification Lens Diffuser

20. Setup Mask Tubes Camera Strobe Light

21. Finish Product Distance to be measured Tube under Backlighting

22. Conclusion Vision systems have unlimited potential Implementation of vision systems will improve the efficiency at various stages of the sequencing process.

23. Acknowledgements Technology Development Group Eva Markiewicz Shailendra Yadav Filip Rege Andrew Barry Todd Sparrow Tracey Honan Pat ___ Directors of Education Shawna Young Dedric Carter Eric Lander Broad Institute