1. Work Package 6: Biomolecular motors on nanostructures Midterm-Review Meeting Molecular Machines- Design and Nano-Scale Handling of Biological Antetypes and Artificial Mimics Specific Targeted Research Project (STREP) in FP6-NMP-2002 Objectives Partners PM Construct kinesin-motor based biological-inorganic hybrid devices for controlled nano-transport processes. ICG-GCTU/d-MBEPFLETH 30 246

2. Midterm-Review Meeting The molecular motors Work Package 6: Biomolecular motors on nanostructures Cytoskeleton motors : Kinesin, Myosin... Microtubules Kinesin f-actin Myosin How do they work ? Energy conversion (M6-1) Structural changes vs. ATP hydrolysis High resolution techniques How include the motors in nano-devices ? (D6-1) Hybrid organic/inorganic systems Nano-handling

3. APD Detection Midterm-Review Meeting Traveling Wave Tracking Work Package 6: Biomolecular motors on nanostructures

4. Single 10 nm step RMS noise ± 2 Å Piezo: 10 nanometers steps Time resolution ~ 2 µs RMS noise ~ 2 Å Drift ~ 1 nm/s Midterm-Review Meeting Traveling Wave Tracking Work Package 6: Biomolecular motors on nanostructures -+ Many motors ? -+ Single motor ?

5. T = 300 K  x = 7.4 pN  s/nm  x = 0.3 pN/nm Average speed = 500 nm/s Backward steps ~ 10 % Step time scale < 70 µs Midterm-Review Meeting Work Package 6: Biomolecular motors on nanostructures

6. Midterm-Review Meeting Structures designed with a 3D approach for the TWT setup (collaboration with the Institut Curie) Glass 3D approach Some samples Work Package 6: Biomolecular motors on nanostructures In order to observe 3D movements, including rotations, the object of study has to be suspended Soft Lithography approach

7. Midterm-Review Meeting Work Package 6: Biomolecular motors on nanostructures Nanostructures for kinesin-driven microtubule motility Rectification, confinement and electrical docking Time sequence of microtubule docking

8. Midterm-Review Meeting Rectification of kinesin-driven microtubule motility SEM image of rectifying structure Fluorescence image of microtubule motility in nanostructures Rectifier geometries 92 % efficiency in rectification Work Package 6: Biomolecular motors on nanostructures

9. Midterm-Review Meeting Specific Targeted Research Project (STREP) in FP6-NMP-2002 Work Package 6: Biomolecular motors on nanostructures Production Van den Heuvel, M. G. L.; Butcher, C. T.; Lemay, S. G.; Diez, S.; Dekker, C. Nano Lett. 2005, 5, 235-241. Van den Heuvel, M. G. L.; Butcher, C. T.; Smeets, R. M. M.; Diez, S.; Dekker, C. Nano Lett. 2005, 5, 1117-1122. L. Busoni, A Dornier, J-L Viovy, J. Prost, G. Cappello; Journal of Applied Physics 2005, 98 A. Dupont, C. Symonds, L. Busoni, J. Prost, G. Cappello; In preparation (2005) M6-1 M6-2 and D6-1 Many different nanofabricated structures were made in TU-Delft New flexible optical technique, called Travelling Wave Tracking (ICG-GC). Microtubules injected in the structures (TU-Delft) and the activity has successfully been checked First experiments on single molecular motor Resources Post-Docs: Irene Dujovne, Clémentine Symonds, Lorenzo Busoni Conferences and Meetings additional projects New motors: Myosin V and VI (collaboration with J. Spudich, Stanford) Gold nanoprobes (collaboration with R. Levy, University of Liverpool) DNA associated motors Conclusion Objectives for the first part of the project have been achieved and we can begin measuring single molecular motors moving on the suspended microtubules.