1. ELISA Testing

2. What is ELISA Technology? Enzyme-Linked Immunosorbant Assay

3. ELISA Testing What is the between Antigen and Antibody testing? Which does an ELISA test use? BOTH

4. The Assay ELISA assays use antibodies that are covalently linked ("conjugated") to enzymes. Antigen is bound to a plastic well, and enzyme-linked antibody is bound to the antigen. Unbound antibody is rinsed away. The amount of enzyme remaining, and hence the amount of antibody bound, is reported by adding a substrate that changes color when acted upon by the enzyme. The absorbance of the resulting color is proportional to the amount of enzyme bound to the well, hence to the amount of antibody, hence to the amount of antigen. Thus, color reports antigen, quantitatively.

5. ELISA Testing Well Tests – Commercial laboratory settings. –Handle large amount of samples –Lower cost –Labor intensive

6. ELISA Testing Well Testing –Heartworm Wells (IDEXX, Synbiotic) –Feline Leukemia (Labs) –HIV –Immunology –Seed Purity

7. ELISA Testing Rapid Assays – In-clinic or patient side testing –Immediate results –Fewer steps –Higher cost

8. ELISA Testing Rapid Assays –Heartworm –FeLV/FIV –Parvo –Pregnancy

9. IDEXX ELISA Tests Snap ® Well Heartworm Bovine Spongiform Encephalopathy Lyme/ E.canis Chronic Wasting Disease Parvo Classic Swine Fever Virus FeLV/ FIV Avian Influenza Giardia Avian Mycoplasma Foal IgG Equine Infectious Anemia Feline Heartworm Lyme C-6 Antibody

10. IDEXX Snap ® Device How it works….

11. IDEXX Snap ® Device How it works…. This is why it is called “Snap”

12. IDEXX Snap ® Device How it works….

13. IDEXX Snap ® Device How it works….

14. IDEXX Snap ® Device Things to remember when running an IDEXX Snap ® Test. –Know how many drops of blood and how many drops of conjugate. –4 Whole blood, serum, or plasma –3 Drops conjugate Standardized for all Snap Tests

15. IDEXX Snap ® Device Things to remember when running an IDEXX Snap ® Test. –“Snap” the device –Does the test kit require refrigeration?

16. Questions?