1. Biological Membranes

2. Lipid Bilayer Phospholipids form bilayers in water Fluid mosaic model of membrane structure Membrane is only 10 nm thick Proteins move around laterally like icebergs in the ocean

3. Hydrophilic Hydrophobic AmphipathicAmphipathic

5. Bilayer Phospholipids form bilayers because the molecules…. –Have two distinct regions, one strongly hydrophobic and the other strongly hydrophilic –Have cylindrical shapes that allow them to associate with water most easily as a bilayer structure

11. Fluid Mosaic Model Singer and Nicholson in 1972 Phospholipid bilayer with proteins embedded within the membrane or otherwise associated with the membrane Dynamic layer- molecules move about laterally in the membrane

14. Membranes are two-dimensional fluids  Molecules move laterally  Phospholipids can rotate  But stay within their layer

15. Frye and Edidin (1970) Fused and labeled human and mouse cells and noted that the some of the molecules in the cell membrane had indeed moved

16. Michael Edidin Graduate Professor at Johns Hopkins University in Baltimore, Maryland

18. Cell Membrane Fluidity To maintain proper function, a membrane’s lipids must be in optimum fluidity Too cold– a problem- could turn solid Too hot- a problem- could make membrane weak

19. Hydrocarbon Chains Chains can twist at the single bond sites This twisting increases as T increases

20. Solutions?? Develop some more unsaturated fats –How would this help? unsaturate d saturated

21. Solutions?? Get some cholesterol! –How would this help? cholesterol phospholipid

22. Plant Cells In plant cells, other steroids perform the same function as cholesterol

23. Exocytosis The cell can create a vesicle that fuses with the membrane and then releases its contents outside of the cytosol

24. Endocytosis The cell can take in nutrients by enclosing them in a vesicle and releasing the vesicle to the cytosol

25. Membrane Proteins Integral Proteins- firmly bound to the membrane Amphipathic –Hydrophilic regions extend outside the cell –Hydrophobic regions intermingle with the fatty acid chains of the phospholipids

27. Transmembrane Protein Extend all the way through the cell membrane Some go through only once and some go through as many as 24 times The most common is  -helix with hydrophobic amino acid side chains

29. Peripheral Proteins Located on the inner or outer surface of the membrane Usually bound to exposed parts of the integral proteins

32. Placement of Proteins in the Membrane As we know, there are proteins embedded in the membrane These proteins are not randomly placed in this location –They have a specific orientation with the membrane There may be more proteins on the inside than the outside and vice versa As we know, there are proteins embedded in the membrane These proteins are not randomly placed in this location –They have a specific orientation with the membrane There may be more proteins on the inside than the outside and vice versa

34. Membrane Protein Formation Inner surface proteins –Manufactured by free ribosomes –Move to the membrane through cytoplasm and fuse with the membrane

35. Membrane Protein Formation Outer Surface Proteins –Formed by ribosomes on the rough ER

36. Membrane Protein Formation Outer Surface Proteins –Become associated in ER membrane and push through into the lumen of the ER Here sugars are added that are found only in the lumen of the ER This makes them glycoproteins protein carbohydrate

37. Membrane Protein Formation Outer Surface Proteins –Transport vesicles move the newly formed gylcoprotein to the golgi complex Golgi complex

38. Membrane Protein Formation Outer Surface Proteins –Glycoprotein becomes associated with the golgi complex in the same orientation as it did with the ER Further modification is made to the carbohydrate while it is in the lumen of the the golgi complex GC may also sort glycoproteins together

39. Membrane Protein Formation Outer Surface Proteins –GC buds and sends vesicle to the plasma membrane where it fuses with the membrane

40. Membrane Protein Formation Outer Surface Proteins –The carbohydrate part of the glycoprotein extends to the outside of the membrane

41. Outer Surface Proteins –Formed by ribosomes on the rough ER –Become associated in ER membrane and push through into the lumen of the ER Here sugars are added that are found only in the lumen of the ER This makes them glycoproteins –Transport vesicles move the newly formed gylcoprotein to the golgi complex –Glycoprotein becomes associated with the golgi complex in the same orientation as it did with the ER Further modification is made to the carbohydrate while it is in the lumen of the the golgi complex GC may also sort glycoproteins together –GC buds and sends vesicle to the plasma membrane where it fuses with the membrane –The carbohydrate part of the glycoprotein extends to the outside of the membrane

42. How do Membranes Fuse? Golgi complex membrane is of same structure as the plasma membrane Fusion is like an air bubble with a speck of dirt in it rising to the surface of a glass of water and depositing the speck on the surface of the water as the water surrounding the bubble becomes part of the water’s surface.

43. Notice any similarities?

44. Membrane Protein Functions