1. The Cellular Level of Organization

2. General Cell Structures Plasma membrane or cell membrane - forms the cell’s outer boundary - separates the cell’s internal environment from the outside environment - is a selective barrier (selective permeability) - plays a role in cellular communication Cytoplasm - everything between the plasma membrane and the nucleus - cytosol: fluid portion - organelles: subcellular structures with specific functions Nucleus - large organelle that chromosomes, each of which consists of a single molecule of DNA and associated proteins - a chromosome contains thousands of hereditary units called genes

3. Typical Cell

4. Plasma Membrane Animation: Membrane FunctionsMembrane Functions

5. Selective Permeability of Membrane Lipid bilayer –permeable to nonpolar, uncharged molecules oxygen, CO 2, steroids –permeable to water which flows through gaps that form in hydrophobic core of membrane as phospholipids move about Transmembrane proteins act as specific channels –small and medium polar & charged particles Macromolecules unable to pass through the membrane –vesicular transport

6. Membrane Proteins

7.  Ion channels  Carriers  Receptors

8. Membrane Proteins  Enzymes  Linkers  Cell identity markers

9. Transport Across the Membrane Diffusion

10. Transport Across the Membrane Diffusion

11. Facilitated Diffusion Channel-Mediated Diffusion Transport Across the Membrane

12. Carrier-Mediated Diffusion Transport Across the Membrane

13. Osmosis

14. Effects of Tonicity

15. Active Transport

16. Secondary Active Transport Antiporters and Symporters

17. Copyright © John Wiley & Sons, Inc. All rights reserved. Transport in Vesicles  Vesicle - a small spherical sac formed by budding off from a membrane  Endocytosis - materials move into a cell in a vesicle formed from the plasma membrane three types: receptor-mediated endocytosis phagocytosis bulk-phase endocytosis (pinocytosis)  Exocytosis - vesicles fuse with the plasma membrane, releasing their contents into the extracellular fluid  Transcytosis - a combination of endocytosis and exocytosis

18. Receptor- Mediated Endocytosis

19. Phagocytosis

20. Endocytosis Bulk-phase endocytosis

21. Copyright © John Wiley & Sons, Inc. All rights reserved. Transport  Transport Across the Plasma Membrane Transport Across the Plasma Membrane Animation

22. Transport of Materials

23. Cytoplasm

24. Copyright © John Wiley & Sons, Inc. All rights reserved. Cytoplasm - 2 Components 1. Cytosol - intracellular fluid, surrounding the organelles - The site of many chemical reactions - Energy is usually released by these reactions. - Reactions provide the building blocks for cell maintenance, structure, function and growth. 2. Organelles - Specialized structures within the cell

25. Cytoskeleton Network of protein filaments throughout the cytosol Provides structural support for the cell Microfilaments –thinnest filaments (actin) –locomotion & division –support microvilli Intermediate filaments –several different proteins –anchor organelles Microtubules –large cylindrical structures (tubulin) –flagella, cilia & centrosomes

26. Copyright © John Wiley & Sons, Inc. All rights reserved. Centrosome  located near the nucleus, consists of two centrioles and pericentriolar material

27. Structure –pairs of microtubules –covered by cell membrane –basal body is centriole responsible for initiating its assembly Differences –cilia short and multiple –flagella longer and single Cilia and Flagella

28. Copyright © John Wiley & Sons, Inc. All rights reserved. Cilia and Flagella

29. Ribosomes sites of protein synthesis

30. Endoplasmic Reticulum - network of membranes in the shape of flattened sacs or tubules - Rough ER - connected to the nuclear envelope, a series of flattened sacs, surface is studded with ribosomes, produces various proteins -Smooth ER - a network of membrane tubules, does not have ribosomes, synthesizes fatty acids and steroids, detoxifies certain drugs

31. 3-20 flattened, curved membranous sacs called cisterns Convex side faces ER & concave side faces cell membrane Processes & packages proteins produced by rough ER Golgi Complex

32. Packaging by Golgi Complex

33. Lysosomes Membranous vesicles formed in Golgi complex filled with digestive enzymes digest foreign substances

34. Peroxisomes –smaller than lysosomes –form by division of preexisting peroxisomes –contain enzymes that oxidize organic material –part of normal metabolic breakdown of amino acids and fatty acids –oxidizes toxic substances such as alcohol and formaldehyde –contains catalase which decomposes H 2 O 2 Proteasomes –continuously destroy unneeded, damaged, or faulty proteins, found in the cytosol and the nucleus

35. Double membrane organelle –central cavity known as matrix –inner membrane folds known as crista surface area for chemical reactions of cellular respiration Function –generation of ATP –“powerhouse of cell” Mitochondria

36. Nucleus Nucleolus –spherical, dark bodies within the nucleus (no membrane) –site of ribosome assembly Large organelle with double membrane nuclear envelope outer membrane continuous with rough ER perforated by water- filled nuclear pores (10X channel pore size)

37. Cell Parts and Their Functions

38. Protein Synthesis

39. TranscriptionTranslation

40. Protein Elongation

41. Cell Cycle

42. DNA Replication

43. Mitosis and Cytokinesis

44. Somatic Cell Cycle

45. Meiosis

46. Mitosis vs. Meiosis

47. Cellular Diversity The average adult has nearly 100 trillion cells. There are about 200 different types of cells. Cells come in a variety of shapes and sizes. Cellular diversity permits organization of cells into more complex tissues and organs.