1. LAB ONE Diffusion and Osmosis

2. Background Concepts: diffusion & osmosis selectively permeable membranes water potential molarity LAB ONE Diffusion and Osmosis

3. After doing this lab, you should be able to: LAB ONE Diffusion and Osmosis

4. After doing this lab, you should be able to: Measure the water potential of a solution in a controlled experiment. LAB ONE Diffusion and Osmosis

5. After doing this lab, you should be able to: Determine the osmotic concentration of living tissue or an unknown solution from experimental data. LAB ONE Diffusion and Osmosis

6. After doing this lab, you should be able to: Describe the effects of water gain or loss in animal and plant cells. LAB ONE Diffusion and Osmosis

7. After doing this lab, you should be able to: Relate osmotic potential to solute concentration and water potential. LAB ONE Diffusion and Osmosis

8. EXERCISE 1A: Diffusion. LAB ONE Diffusion and Osmosis

9. EXERCISE 1A: Diffusion dialysis tubing LAB ONE Diffusion and Osmosis beaker

10. EXERCISE 1A: Diffusion dialysis tubing LAB ONE Diffusion and Osmosis beaker 15% glucose 1% starch

11. EXERCISE 1A: Diffusion dialysis tubing LAB ONE Diffusion and Osmosis beaker 15% glucose 1% starch distilled water

12. EXERCISE 1A: Diffusion dialysis tubing LAB ONE Diffusion and Osmosis beaker 15% glucose 1% starch distilled water Iodine (Lugol’s)

13. EXERCISE 1A: Diffusion LAB ONE Diffusion and Osmosis

14. EXERCISE 1A: Diffusion LAB ONE Diffusion and Osmosis 30 min.

15. EXERCISE 1A: Diffusion LAB ONE Diffusion and Osmosis 30 min.

16. EXERCISE 1A: Diffusion LAB ONE Diffusion and Osmosis Glucose test

17. EXERCISE 1A: Diffusion LAB ONE Diffusion and Osmosis Glucose test positive

18. EXERCISE 1A: Diffusion LAB ONE Diffusion and Osmosis Glucose test positive

19. EXERCISE 1B: Osmosis LAB ONE Diffusion and Osmosis dialysis bags

20. EXERCISE 1B: Osmosis LAB ONE Diffusion and Osmosis distilled0.2 M0.4 M 0.6 M 0.8 M1.0 M sucrose solutions of various molarities

21. EXERCISE 1B: Osmosis LAB ONE Diffusion and Osmosis distilled0.2 M0.4 M 0.6 M 0.8 M1.0 M 26.0 g 26.2 g 26.1 g 26.4 g 26.3 g 26.4 g

22. EXERCISE 1B: Osmosis LAB ONE Diffusion and Osmosis 26.0 g 26.2 g 26.1 g 26.4 g 26.3 g 26.4 g distilled water

23. EXERCISE 1B: Osmosis LAB ONE Diffusion and Osmosis 26.0 g 26.2 g 26.1 g 26.4 g 26.3 g 26.4 g 30 min.

24. EXERCISE 1B: Osmosis LAB ONE Diffusion and Osmosis distilled0.2 M0.4 M 0.6 M 0.8 M1.0 M 26.0 g 26.2 g 26.1 g 26.4 g 26.3 g 26.4 g gently blot dry

25. EXERCISE 1B: Osmosis LAB ONE Diffusion and Osmosis distilled0.2 M0.4 M 0.6 M 0.8 M1.0 M 26.0 g 26.2 g 26.1 g 26.4 g 26.3 g 26.4 g 26.3 g 27.0 g 28.1 g 29.3 g 30.2 g 31.2 g

26. EXERCISE 1C: Water Potential LAB ONE Diffusion and Osmosis

27. EXERCISE 1C: Water Potential LAB ONE Diffusion and Osmosis potato

28. EXERCISE 1C: Water Potential LAB ONE Diffusion and Osmosis

29. EXERCISE 1C: Water Potential LAB ONE Diffusion and Osmosis potato cubes

30. EXERCISE 1B: Osmosis LAB ONE Diffusion and Osmosis 0.2 M 0.4 M 0.6 M 0.8 M 1.0 M sucrose solutions of various molarities distilled

31. EXERCISE 1B: Osmosis LAB ONE Diffusion and Osmosis

32. EXERCISE 1B: Osmosis LAB ONE Diffusion and Osmosis overnight

33. EXERCISE 1B: Osmosis LAB ONE Diffusion and Osmosis overnight

34. EXERCISE 1B: Osmosis LAB ONE Diffusion and Osmosis overnight

35. EXERCISE 1B: Osmosis LAB ONE Diffusion and Osmosis overnight

36. EXERCISE 1B: Osmosis LAB ONE Diffusion and Osmosis overnight

37. EXERCISE 1B: Osmosis LAB ONE Diffusion and Osmosis

38. EXERCISE 1E: Plasmolysis MATERIALS An onion A microscope 15 % NaCl solution (a few drops) Distilled water. LAB ONE Diffusion and Osmosis

39. EXERCISE 1E: Plasmolysis PROCEDURE Observe onion cells @ 100x Flood with NaCl and observe Flood with distilled H 2 O and observe. LAB ONE Diffusion and Osmosis