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Published byOswald Casey Modified over 8 years ago
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B C Supplementary Figure 1 Control X0 Control X9 -HBx (mAb) -HBx (rabbit serum) ABCEFD 1 58 85 120 154 aa ABCEF X0 X9 ( D) A 100 90 80 70 60 50 40 30 20 10 0 0 1 2 3 4 6 8 10 Control X0 X9 Control X0 X9 Control X0 X9 Control X0 X9 Control X0 X9 Control X0 X9 Control X0 X9 Control X0 X9 Post Double Thymidine Block (h) Cells (%)
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Cyclin A Binding (68-108) DNA Binding (120-191) DP-1 Binding (206-283) Transactivation Domain (323-437) Rb binding E2F1-WT E2F1 (1-374) Transactivation Domain mutant Wild-type C B Normalized luciferase units Control X0 pGL3 CDC6 WT CDC6-SM1 # # A + 225 + 1 - 100 E2F1 CCAAT NF - 200 AP-2 -1534 CDC6 WT Wild type E2F1 binding site TTTGGCGC E2F1 Mutant E2F1 binding site TTTAACGC CDC6-SM1 E2F1 -130 Luciferase CDC6 promoter [-1534, +225] Supplementary Figure 2
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E2F1 - 242 E2F1 - 242 pGL3-E2F1 WT pGL3-E2F1 Mut Luciferase + 127 - 122 E2F1 CCAAT Sp1 - 204 -728 CCAAT Sp1 - 242 + 1 E2F1 promoter [-728, +127] Supplementary Figure 3
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Relative abundance of CDC6 on human -globin origin of replication (arbitrary unit) Serum Stimulation (h) Relative abundance of CDC6 on human -globulin locus (arbitrary unit) Serum Stimulation (h) ChIP -CDC6 Control Input Serum Stimulation Control X0 X9 A 3h 7h Control ChIP Input Serum Stimulation Control X0 X9 B 3h 7h -CDC6 Human -globin origin -globulin non-origin locus Supplementary Figure 4
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