1. MCB 186 CIRCADIAN BIOLOGY Clock genes & Biochemical Mechanisms: Kai genes October 10, 2007 J. W. Hastings

2. CORE CLOCK COMPONENTS IN FEEDBACK LOOPS OF 3 SYSTEMS

3. TEMPORAL SEPARATION OF CELL BIOCHEMICAL ACTIVITIES IN CYANOBACTERIA ( OXYGEN, NITROGENASE ETC) MITSUI ET AL 1986

4. CIRCADIAN RHYTHM of CYANOBACTERIAL GENE mRNA Kondo et al. 1993 PNAS LUCIFERASE REPORTER

5. Turntable Screening Apparatus: 12 positions for petri dishes or titer plates

6. BACTERIAL COLONIES EXPRESSING BIOLUMINESCENCE Kondo et al. 1993 PNAS

7. Cyanobacterial in vivo Rhythm with Bacterial Luciferase as a Reporter Luminescence Kondo, Johnson Golden et al., 1993, PNAS

8. EFFECT OF DARK EXPOSURES ON PERIOD In LL during ---- In light but with 3 min in dark every 30 min

9. DARK PULSE PHASE SHIFTS IN CYANOBACTERIA KONDO ET AL 1993

10. TEMPERATURE has SMALL EFFECT on PERIOD of BACTERIAL RHYTHM

11. PERIOD MUTANTS OF CYANOBACTERIA KONDO ET AL.1995

12. CYANOBACTERIA ARHYTHYMIC & AMPLITUDE MUTANTS KONDO 1995

13. PERIOD VARIATION OF INDIVIDUAL CYANOBACTERIA MUTANT CLONES KONDO ET AL.1995

14. LUCIFERASE REPORTER RANDOMLY INSERTED IN FRONT OF PROMOTERS IN BACTERIA. ABOUT 800 LUMINOUS COLONIES ISOLATED ALL EXHIBITED CIRCADIAN RHYTHMS ACROPHASES NOT ALL THE SAME

15. ACROPHASE CLASSES OF CLOCK-CONTROLLED GENES LIU ET AL,1995

16. KAI genes in SYNECHOCOCCUS and MUTANT MAPPING ISHIUR

17. PHENOTYPES OF KAI GENE INACTIVATIONS ISHIURA ET AL 1998

18. EXPRESSION OF KAI GENE CONSTRUCTS ISHIURA ET AL 1998

19. Cyanobacterial Clockworks TTO Model - 1998 Ishiura et al., 1998

20. Cyanobacterial Clockwork Model TRANSCRIPTIONAL CONTROL OF MANY GENES 2003 Xu et al., EMBO J. 22: 2117-2126, 2003

21. Kai C PHOSPHORYLATION RHYTHM in vitro in a SOLUTION CONTAINING only ATP with Kai A, B and C Phase is not determined by light Nakajima et al, Science, 308: 414-415 (2005 )

22. IN VITRO CIRCADIAN RHYTHM KaiC PHOSPHORYLATION

23. In vitro phosphorylation rhythm is temperature compensated Nakajima, M et al Kondo, T (2005) Reconstitution of circadian oscillation in vitro Science 308: 414-415

24. In vitro phosphorylation rhythm is temperature compensated Nakajima, M et al Kondo, T (2005) Reconstitution of circadian oscillation in vitro Science 308: 414-415

25. Period of phosphorylation rhythm corresponds to mutant period Tomita, Nakajima, Kondo & Iwasaki (2005) Science 307: 251-254

26. Tau of in vitro P-oscillation corresponds to mutant tau Nakajima, M et al Kondo,T (2005) Reconstitution of circadian oscillation in vitro Science 308: 414-415

27. Simplified model for the posttranslational oscillator Nakajima, M et al Kondo,T (2005) Science 308: 414-415

29. EVIDENCE THAT TRANSCRIPTION and TRANSLATION are not NECESSARY for CIRCADIAN OSCILLATIONS IN CYANOBACTERIA Tomita, Nakajima, Kondo & Iwasaki (2005) No transcription-translation feedback in circadian rhythm of KaiC phosphorylation Science 307: 251-254

30. NO RHYTHM OF kaiA or kaiBC mRNAs in DD Tomita, Nakajima, Kondo & Iwasaki (2005) No transcription-translation feedback in circadian rhythm of KaiC phosphorylation Science 307: 251-254

31. NO RHYTHM OF KaiC PROTEIN in DD Tomita, Nakajima, Kondo & Iwasaki (2005) Science 307: 251-254

32. RHYTHM OF KaiC PROTEIN PHOSPHORYLATION in DD Tomita, Nakajima, Kondo & Iwasaki (2005) Science 307: 251-254

33. Period Temperature Compensation of in vivo Rhythm of KaiC Phosphorylation in DD Tomita, Nakajima, Kondo & Iwasaki (2005) No transcription-translation feedback in circadian rhythm of KaiC phosphorylation Science 307: 251-254

34. Model for the posttranslational oscillator coupled with TTO Tomita, Nakajima, Kondo & Iwasaki (2005) No transcription-translation feedbackin circadian rhythm of KaiC phosphorylation Science 307: 251-254

35. Tau of in vitro P-oscillation corresponds to mutant tau Nakajima, M et al Kondo, T (2005) Reconstitution of circadian oscillation in vitro Science 308: 414-415