1. Lab. Safety, Microscopes and Colony Morphology Supplemental instruction Picture from http://www.toonpool.com/cartoons/MICROSCOPE%20LOOKING_27378 http://infectionnet.org/supporting/candida-plate/http://www.toonpool.com/cartoons/MICROSCOPE%20LOOKING_27378http://infectionnet.org/supporting/candida-plate/ Designed by Pyeongsug Kim ©2010 webmaster@science-i.comwebmaster@science-i.com Fall 2011 Chattahoochee Technical College

2. Designed by Pyeongsug Kim, ©2010 www.science-i.comwww.science-i.com Picture from http://nobel.scas.bcit.ca/debeck_pt/science/safety.htm www.google.com/imageshttp://nobel.scas.bcit.ca/debeck_pt/science/safety.htmwww.google.com/images Lab. Safety Food and Drink Not allowed. Safety goggles, Please…. Dispose materials in proper place.

3. Designed by Pyeongsug Kim, ©2010 www.science-i.comwww.science-i.com Dispose materials solid Bacteria containing solid materials. To biohazard can. Microscopic slides To glass disposal box Picture from http://electronmicroscopy.org/Histology_Disposal.htm http://www.globescientific.com/20-21mm-40place-plastic-test-tube-rack-p-1045.htmlhttp://electronmicroscopy.org/Histology_Disposal.htmhttp://www.globescientific.com/20-21mm-40place-plastic-test-tube-rack-p-1045.html liquid Bacteria containing liquid test tubes. To waste rack

4. Designed by Pyeongsug Kim, ©2010 www.science-i.comwww.science-i.com Picture from http://www.2spi.com/catalog/supp/parafilm.php https://mycotopia.net/forums/agar-strain-isolation/43307-agar-plates.htmlhttp://www.2spi.com/catalog/supp/parafilm.phphttps://mycotopia.net/forums/agar-strain-isolation/43307-agar-plates.html Dispose agar plates In order to dispose agar plate, wrap the plate with lab. film before putting the biohazard can.

5. What type of microscope are you seeing? Light compound microscope In which object do you need immersion oil to see the bacterial sample? 100X objective Why do you need oil-immersion? (how the oil-immersion work?) Bacteria is too small; Oil – to increase the magnification and to remove light refraction and to improve resolution. Designed by Pyeongsug Kim, ©2010 www.science-i.comwww.science-i.com

6. D C A B What are the names of these parts? Write each function. E The answer is in your textbook or lab. notebook. Designed by Pyeongsug Kim, ©2010 www.science-i.comwww.science-i.com

7. 10 X 40 X 100X 40 X 10 X 100X Total magnification on the slide in view? You want to see in 600 times magnification. Which part do you change to? (turn into..) 100x 400x 60x objective Designed by Pyeongsug Kim, ©2010 www.science-i.comwww.science-i.com

8. Designed by Pyeongsug Kim, ©2010 www.science-i.comwww.science-i.com Colony Morphology Picture from http://inst.bact.wisc.edu/inst/index.php?module=book&func=displayarticle&art_id=119http://inst.bact.wisc.edu/inst/index.php?module=book&func=displayarticle&art_id=119

9. Colony Morphology and Arrangements Designed by Pyeongsug Kim, ©2010 www.science-i.comwww.science-i.com -Cocci DiplococcusDiplococcus: cocci arranged in pairs. StreptococcusStreptococcus: cocci arranged in chains Staphylococcus:Staphylococcus: cocci arranged in irregular, often grape-like clusters Diplococcus Picture from http://faculty.ccbcmd.edu/courses/bio141/lecguide/unit1/shape/diplo.html http://www.flashcardmachine.com/microbiology-lab-quizncc.htmhttp://faculty.ccbcmd.edu/courses/bio141/lecguide/unit1/shape/diplo.htmlhttp://www.flashcardmachine.com/microbiology-lab-quizncc.htm Staphylococcus Streptococcus

10. Colony Morphology and Arrangements Designed by Pyeongsug Kim, ©2010 www.science-i.comwww.science-i.com -Bacillus(Rods) BacillusBacillus: a single bacillus DiplobacillusDiplobacillus: Bacilli that remain in pairs after they divide. StreptobacillusStreptobacillus: bacilli in chains CoccobacillusCoccobacillus: oval and similar to a coccus Picture from http://faculty.ccbcmd.edu/courses/bio141/lecguide/unit1/shape/diplo.html http://faculty.ccbcmd.edu/courses/bio141/labmanua/lab1/lab1.htmlhttp://faculty.ccbcmd.edu/courses/bio141/lecguide/unit1/shape/diplo.htmlhttp://faculty.ccbcmd.edu/courses/bio141/labmanua/lab1/lab1.html Bacilli Streptobacilli

11. Designed by Pyeongsug Kim, ©2010 www.science-i.comwww.science-i.com Picture from http://classes.midlandstech.edu/carterp/courses/bio225/chap04/lecture2.htmhttp://classes.midlandstech.edu/carterp/courses/bio225/chap04/lecture2.htm