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Published byBeverly Stokes Modified over 8 years ago
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Gihan E-H Gawish, MSc, PhD Ass. Professor Molecular Genetics and Clinical Biochemistry Molecular Genetics and Clinical BiochemistryKSU 9 TH WEEK
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After replication, each DNA molecule= one old strand and one new strand. After replication, each DNA molecule= one old strand and one new strand.
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Three possible models Correct
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Watson and Crick’s structure predicted that base-pairing would guide semi-conservative replication. Matthew Meselson and Franklin Stahl tested this prediction by labeling DNA with 15N (heavy isotope). Watson and Crick’s structure predicted that base-pairing would guide semi-conservative replication. Matthew Meselson and Franklin Stahl tested this prediction by labeling DNA with 15N (heavy isotope).
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Animation
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Click to find the attached file Click to Exam Yourself Click to Exam Yourself click to solve the quiz in this animation DNA Replication Fork Animation
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3’OH of growing chain forms covalent bond with phosphate of dNTP. DNA polymerases can only add nucleotides to 3’ end (5’—>3’ direction of synth.
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1) Template to specify complementary strand. 2) Primer (DNA or RNA) bound to template by base pairing. No DNA pol. can start synth. without a primer!! 3) dNTPs
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Heat to 95C 5’-GATCTTAGC-3’ 3’-CTAGAATCG-5’ Cool to 70 C When cooled, base-pairs find each other and become double-stranded (renature, reanneal, or hybridize). 5’-GATCTTAGC-3’ 3’-CTAGAATCG-5’ DNA can be denatured and reannealed:
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Uses DNA replication to amplify tiny amounts of DNA to give an amount large enough to be analyzed. Requires: 1) Small amount of template. 2) Specific primers that bind the template. 3) dNTPs. 4) A thermostable DNA polymerase. Uses DNA replication to amplify tiny amounts of DNA to give an amount large enough to be analyzed. Requires: 1) Small amount of template. 2) Specific primers that bind the template. 3) dNTPs. 4) A thermostable DNA polymerase.
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