1. Environmental Microbiology (MLEM-201) What do Microbes do? How can we use this to our advantage? Lecturer: Dr. Mohamed Salah El-Din

2. Intended Learning Outcomes (ILO’s): Student will be able: To achieve a basic understanding of aquatic microbiology and microbial processes To know the major microorganisms of importance in freshwater, seawater, and groundwater To understand the major microbial processes involved in those habitats To understand the major environmental factors and influences in those habitats To understand how to apply basic microbiological and ecological principles in understanding the applied processes known as bioremediation of contaminated soils and groundwater.

3. Bioremediation Use of microorganism to remove pollution Cheaper Can use natural organism Is helped by preventing limited nutrients

4. Solid Municipal Waste piles Many municipal waste piles are inefficiently run because they are dry and anaerobic

5. Aquatic conditions Biofilms are composed of whole communities of microbes that are metabolically diverse Bodies of water are naturally set up to process waste Tend to grow in presence of oxygen and light Use is best when oxygen content is increased Phytoplankton in oceans are primary producers in the open ocean

6. Freshwater Donation Figure 27.12

7. Role of microorganism in water quality Biomagnifications Indicators of fecal contamination Blooms Eutrophication

8. Waterborne Diseases Table 27.2

9. Water quality tests Coliforms are aerobic or facultatively anaerobic, gram negative non endospore forming rods that ferment lactose with the production of acid and gas within 48 hours of been placed in a medium at 35’C Fecal Coliforms predominantly E. coli are used to indicate the presence of human fecies

10. Aerobic or facultatively anaerobic, gram- negative, non–endospore forming rods that ferment lactose to acid + gas within 48 hr, at 35°C Indicator organisms –Used to detect fecal contamination MPN –Most probable number/100 ml of water Coliforms

11. Assignment: As a part of the semester activity, one student is selected every week to prepare a short seminar about his/her point of interest in one of the lecture topics. That to be discussed and evaluated during the next lecture.