1. A Unified Approach to Human Embryo Culture
An Overview of LifeGlobal Media
A Unified Approach to Human Embryo Culture
Don Rieger PhD and Klaus Wiemer Ph.D.
Life Global, Northwest Center for Reproductive
Sciences
Don Rieger - June, 2006

2. An Overview of LifeGlobal Media
A Single Medium
vs.
Sequential Media
from Zygote to Blastocyst
Don Rieger - June, 2006

3. A Single Medium vs. Sequential Media for Embryo Culture
An Overview of LifeGlobal Media
A Single Medium vs. Sequential Media for Embryo Culture
“… in order to optimize mammalian embryo development in culture, sequential media are required, each designed to meet the changing requirements of the developing embryo.”
(Gardner and Lane, In Principles of Cloning, , 2002)
“The popularity of this conviction is due to its intuitive appeal, but critical examination shows that it is not supported by direct experimental evidence”
(Biggers et al., Hum Reprod 20, , 2005)
Don Rieger - June, 2006

4. An Overview of LifeGlobal Media
A single medium can be used for culture from the zygote to the blastocyst stage
Changing the medium is a stress on the embryo
Don Rieger - June, 2006

5. An Overview of LifeGlobal Media
The Development of global®
Medium
Year
Investigators
SOM
1991
Lawitts & Biggers
KSOM
1994
Erbach et al.
KSOMaa
2000
Biggers et al.
global® (Ala-Gln)
2002
Wiemer et al.
global® (Gly-Gln)
2006
(Biggers et al., 2004)
Don Rieger - June, 2006

6. An Overview of LifeGlobal Media
global® Formulation
An Overview of LifeGlobal Media
Don Rieger - June, 2006

7. An Overview of LifeGlobal Media
The global® Family
of Media
Don Rieger - June, 2006

8. An Overview of LifeGlobal Media
The global® Family of Media
An Overview of LifeGlobal Media
Objective
To maintain, as closely as possible, the same medium throughout all stages of gamete and embryo handling and culture
In order to
Minimize the stress of changing from one medium to another
Don Rieger - June, 2006

9. An Overview of LifeGlobal Media
1. global® for Fertilization
Don Rieger - June, 2006

10. An Overview of LifeGlobal Media
Energy Metabolism
of the COC and Sperm
An Overview of LifeGlobal Media
Don Rieger - June, 2006

11. An Overview of LifeGlobal Media
global® for Fertilization Formulation
An Overview of LifeGlobal Media
Don Rieger - June, 2006

12. An Overview of LifeGlobal Media
2. global® w/HEPES
Don Rieger - June, 2006

13. An Overview of LifeGlobal Media
Acid-Base Buffer Systems
An Overview of LifeGlobal Media
Don Rieger - June, 2006

14. An Overview of LifeGlobal Media
global® w/HEPES Formulation
An Overview of LifeGlobal Media
Don Rieger - June, 2006

15. An Overview of LifeGlobal Media
3. PGD Biopsy Medium
Don Rieger - June, 2006

16. An Overview of LifeGlobal Media
The PGD Biopsy Procedure
An Overview of LifeGlobal Media
Don Rieger - June, 2006

17. An Overview of LifeGlobal Media
The Effect of Biopsy Medium
on Pregnancy Rate
An Overview of LifeGlobal Media
(Hill & Li, The Clinical Embryologist 7, 11-12, 2004)
Don Rieger - June, 2006

18. An Overview of LifeGlobal Media
PGD Biopsy Medium Formulation
An Overview of LifeGlobal Media
Don Rieger - June, 2006

19. An Overview of LifeGlobal Media
The Unified Approach
to Culture
Don Rieger - June, 2006

20. An Overview of LifeGlobal Media
ART Procedures – Day 0
Classical IVF
global® for Fertilization
ICSI
global® w/HEPES
Culture
global®
Day 1
Day 1
Don Rieger - June, 2006

21. An Overview of LifeGlobal Media
ART Procedures – Day 1
Classical IVF
global® for Fertilization
Post-ICSI Culture
global®
Culture
fresh droplets of
global®
Day 3
Don Rieger - June, 2006

22. An Overview of LifeGlobal Media
ART Procedures – Day 3
An Overview of LifeGlobal Media
Culture
global®
Transfer
global®
Cryopreservation
PGD Biopsy
PGD Biopsy Medium
Assisted Hatching
global® w/HEPES
Culture
fresh droplets of
global®
Day 5
Don Rieger - June, 2006

23. An Overview of LifeGlobal Media
ART Procedures – Day 5 and 6
An Overview of LifeGlobal Media
Culture
global®
Transfer
global®
Cryopreservation
Culture
fresh droplets of
global®
Day 6
Transfer
global®
Cryopreservation
Don Rieger - June, 2006

24. An Overview of LifeGlobal Media
Thank You
Don Rieger - June, 2006