1. PAMC: Polar and Alpine Microbial Collection Yung Mi Lee 1, Cheng-Dae Choe 2, Jeong-Han Yim 1, Hong Kum Lee 1, and Soon Gyu Hong 1 1 Korea Polar Research Institute 2 BRN Science Co.

2. Needs for Database ▣ Management and sharing of bio-resources from polar areas ▣ Sharing biodiversity information with national and international organization www. iobis.org

3. Culture Collections No. of Culture collectionsNo. of Cultures Total5851,725,498 755,818 of them are Bacteria. 505,521 of them are Fungi.. www.wfcc.info

4. v PAMC: Polar and Alpine Microbial Collection ▣ pamc.kopri.re.kr pamc.kopri.re.kr ▣ Culture Collection to maintain and distribute microorganisms originated from polar areas ▣ Approximately 500 strains are available for delivery and the number will increase gradually Polar and Alpine Microbial Collection About PAMC

5. Data Management of PAMC PAMC Curators Public databases Public users Search Data download Public users Contributors Public users Contributors Deposit of resources Delivery of resources Long-term preservation of bio-resources Identification Characterization Data updates Metadata upload (AMD) Original data transfer (EBA, SCAR-Marbin etc) Data download (GenBank Taxonomy etc)

6. Strains ▣ Microbial cultures  Bacteria: 6,400 strains  Fungi: 300 strains  Freezing using Glycerol (-180  C) and DMSO (-80  C)  Freeze-dry (4  C) ▣ Preservation Preservation of strains

7. Phylum composition of identified strains Gammaproteobacteria,597 Actinobacteria, 188 Bacteroidetes,191 Firmicutes, 265 Betaproteobacteria,49Alphaproteobacteria,50 Habitats of strains Summary of PAMC Strains Locality distribution of strains Sediment43.70% Soil20.50% Water9.70% Cryoconite8.50% Rock1.10% Glacier/Snow0.30% Biofilm8.50% Algae3.60% Lichen1.30% Debris (animal/plant) 1.20% Animal0.80% Moss0.80%

8. Composition of Identified Strains Actinobacteria 16 Genera 188 Strains Bacteroidetes 25 Genera 191 Strains Firmicutes 11 Genera 265 Strains Alphaproteobacteria 16 Genera 50 Strains Betaproteobacteria 4 Genera 49 Strains G ammaproteobacteria 18 Genera 597 Strains

9. Physiological Characteristics ▲ Growth on the 96 well plate ▲ Formation of hydrolytic zone on the 96 well plate Growth Temperature No. of Strains ProteaseLipase Exopolysaccharide

10. Sample Database

11. Strain Database

12. ① Pseudoalteromonas ② ④ v v v v ③ PAMC - Searching

13. Polar and Alpine Microbial Collection PAMC – Advanced Searching

14. Searching Keywords in PAMC Searching for microbial strains using various keywords such as taxonomy, geography, habitats and physiological characteristics are available in PAMC. TaxonomyGeography Habitats Characteristics Polar and Alpine Microbial Collection

15. Vision of PAMC ▣ To maintain strains for the sustainable use ▣ To supply authentic strains for polar sciences ▣ To expand the service materials: fungal culture, gDNA, metabolite extract, etc ▣ PAMC website will be available on 1 October