1. WP2-P6-INRA LeafyVeg-26November2008 Activity of the P6 in the frame work of the project: regeneration of 300 lettuce accessions WP 2 – P6 (INRA-France) Objectives for year 2 the regeneration of 75 L. sativa in year 1, regenerated seeds of 82 L. sativa have been included in the project

2. WP2-P6-INRA LeafyVeg-26November2008 Work made at INRA in year 2 Regeneration of lettuce accessions Control of the harvested seeds Seed production under insect proof plastic tunnel Storage of seeds at 6°C Uniformity and identity of the progenies Insect-proof tunnel Control of winter cultivars Sample of seeds of the 82 accessions from 2007 Safety duplication at Warwick

3. WP2-P6-INRA LeafyVeg-26November2008 Regeneration of lettuce in 2008 Culture in 2008: 74 accessions (with 2 plants)  Sowing of old seeds22 February  Plantation 26 March  Notation of the minimum descriptors for flowering  Harvesting plant by plant of 69 cv from July to August (> 10 g/plant)  Storage of harvested seeds at 6°C October Insect-proof tunnel used for seed production 4 of the dead accessions in summer regenerated in this fall in a glasshouse

4. WP2-P6-INRA LeafyVeg-26November2008 Regeneration of lettuce in 2008 Tunnel for multiplication in the end of April Angurtana Berenice Arda Barba di Frati Plants on plastic mulch, with stakes Groups of 12 plants, corresponding to 6 accessions with 2 plants per cv

5. WP2-P6-INRA LeafyVeg-26November2008 Regeneration of lettuce in 2008 Tunnel for multiplication in the begining of May Alicia 35 butterhead lettuce Moskovskij

6. WP2-P6-INRA LeafyVeg-26November2008 Regeneration of lettuce in 2008 Tunnel for multiplication in the beginning of May 26 crisp lettuce Red sails Maravilla de Verano

7. WP2-P6-INRA LeafyVeg-26November2008 Regeneration of lettuce in 2008 Tunnel for multiplication in the beginning of May Angurtana Barba di Fratti 6 cos lettuce 3 latin lettuce 4 cutting lettuce

8. WP2-P6-INRA LeafyVeg-26November2008 Culture in field in 2008 : 35 accessions harvested in 2007 Control in 2008 of harvested seeds  Sowing 12 March  Plantation 8 April  Notation of the minimum descriptors for vegetative characters and photosMay Culture in tunnel in 2008: 22 winter accessions harvested in 2008  Sowing 18 September  Plantation 8 October  Notation of the minimum descriptors for vegetative characters and photosNovember-December

9. WP2-P6-INRA LeafyVeg-26November2008 Field in May 2008 Total  for the LeafyVeg project Each accession: 2 plots of 20 plants 1 plot = progeny of one plant harvested in insect-proof tunnel in summer 2007 Other accessions are recent cultivars 27 crisp  11 20 butterhead  13 5 cos  3 15 cutting lettuce  8

10. WP2-P6-INRA LeafyVeg-26November2008 Control of summer lettuce Photos for accession characterisation Butterhead lettuce Crisp lettuce Cos lettuce Cutting lettuce Cv Eynat Cv Radichetta Cv Dorémi Cv Montemar Cv Kinemontepas Cv Kagraner

11. WP2-P6-INRA LeafyVeg-26November2008 Winter tunnel in November 2008 Each accession: 2 plots of 32 plants Other accessions are recent cultivars 11 crisp  7 22 butterhead  15 1 cos 1 cutting lettuce Total  for the LeafyVeg project

12. WP2-P6-INRA LeafyVeg-26November2008 Control of winter Butterhead lettuce Example with an old cultivar Butterhead, cv Blondine Observation of this plot Cultigroup is conform No wrong plant Seed sample is good to include in the European collection

13. WP2-P6-INRA LeafyVeg-26November2008 Control of winter Butterhead lettuce Examples : 2 modern cultivars from breeding companies Batavia, cv Bacarès Butterhead, cv Galore In these plots : Cultigroup is conform No wrong plant Seed samples are good

14. WP2-P6-INRA LeafyVeg-26November2008 Control of winter lettuce Photos for accession characterisation Cv Duran The earliest butterhead Cv BristolCv Isodor Cv PirouetteCv Norden Cv Apollo

15. WP2-P6-INRA LeafyVeg-26November2008  Safety duplication of 82 accessions (sent to Dave on April)  Regeneration of 73 accessions (69 in summer + 4 in fall) and storage of the seeds  Control of harvested seeds in open field = 35 field accessions harvested in 2007 under plastic tunnel = 22 winter accessions harvested in 2008  Notation of minimum descriptors and photos (in progress for winter accessions) Conclusion of the year 2 for P6 Activities made in 2008

16. WP2-P6-INRA LeafyVeg-26November2008  Copy in ILDB file of the minimum descriptors  Make a CD with photos of the plants at the heading stage if the parters are interested to put them on the ILDB Web site Conclusion of the year 2 for P6 Work still to be done Activities planned in LeafyVeg project  Storage at -18°C and safety duplication at Warwick of the seeds harvested in 2008 Other activities

17. WP2-P6-INRA LeafyVeg-26November2008 Project for the year 3 for P6 Activities to made in 2009 At the end of the year 3  Regeneration of 74 accessions and storage of the seeds  Control of harvested seeds  Notation of minimum descriptors and photos 229 regenerated accessions out of the 300 in the project