1. T. Trimpe 2005 http://sciencespot.net/

2. Body Tube Nosepiece Objectives Stage Clips Light Ocular lens (Eyepiece) Arm Stage Coarse Adjustment Fine Adjustment Always carry a microscope with one hand holding the arm and one hand under the base. Base Diaphragm

3. What’s my power? To calculate the power of magnification, multiply the power of the ocular lens by the power of the objective. What are the powers of magnification for each of the objectives we have on our microscopes? Fill in the table on your worksheet.

4. Comparing Powers of Magnification We can see better details with higher the powers of magnification, but we cannot see as much of the image. Which of these images would be viewed at a higher power of magnification?

5. 1. Always carry the microscope with two hands - one on the arm and one underneath the base of the microscope. Hold it up so that it does not hit tables or chairs. Never swing the microscope. 2. Do not touch the lenses. If they are dirty, please raise your hand and ask for the special lens paper to clean the lenses. 3. Be cautious when handling glass slides and cover-slips. Please do not handle broken glass - notify teacher. 4. Always clean slides and microscope when finished. Store microscope set on the lowest objective with the nosepiece turned down to its lowest position (using the coarse adjustment knob). 5. Cover microscope with dust cover and return microscope to storage if so requested. Microscope Safety

6. Let’s give it a try... 1 – Turn on the microscope and then rotate the nosepiece to click the 4X objective into place. 2 – Place a slide on the stage and secure it using the stage clips. Use the coarse adjustment knob (large knob) to get it the image into view slowly moving the knob away from you (stage is moving down). 4 – When you are done, return the nosepiece to the 4X objective the microscope and put up the stage all the way up and return the slides you used. 3 – Once you have the image in view, rotate the nosepiece to view it under different powers. Draw what you see on your worksheet! Be careful with the largest objective! Sometimes there is not enough room and you will not be able to use it!

7. How to make a wet-mount slide … 1 – Get a clean slide and coverslip from your teacher. 2 – Place ONE drop of water in the middle of the slide. Don’t use too much or the water will run off the edge and make a mess! 3 – Place the edge of the cover slip on one side of the water drop. You do not need to use the stage clips when viewing wet-mount slides! 5 – Place the slide on the stage and view it first with the scanning objective. Once you see the image, you can rotate the nosepiece to view the slide with the different objectives. 4 - Slowly lower the cover slip on top of the drop. Cover Slip Lower slowly

8. Tips for Creating A Wet Mount: 1.Gather a thin slice/piece of whatever your specimen is. If your specimen is too thick, then the coverslip will wobble on top of the sample like a see-saw: 2. Place ONE drop of water directly over the specimen. If you put too much water over the specimen, then the coverslip will float on top of the water, making it harder to draw the specimens as they float past the field of view! 3. Place the coverslip at a 45 degree angle (approximately), with one edge touching the water drop, and let go. You don’t want any air bubbles trapped in your slide or in your field of view.

9. Steps for Using the Microscope 1 – Turn on the microscope and then rotate the nosepiece to click the 4X objective into place. 2 – Place a slide on the stage and secure it using the stage clips. Use the coarse adjustment knob to get it the image into view slowly moving the knob away from you (stage is moving down). 4 – When you are done, return the nosepiece to the 4X objective the microscope and put up the stage all the way up and return the slides you used. 3 – Once you have the image in view, rotate the nosepiece to view it under different powers-low power and high power. Draw what you see on your worksheet!

10. How to make a _________________… 1 – Get a clean _________ and ______________from your teacher. 2 – Place ___ drop of water in the middle of the slide. Don’t use too much or the water will run off the edge and make a mess! 3 – Place the ______ of the _________on __ side of the water drop. 5 – Place the slide on the stage and view it first with the _________objective. Once you see the image, you can rotate the nosepiece to view the slide with the different objectives. 4 - __________ lower the cover slip on top of the drop. Cover Slip Lower slowly