1. 1 / 9 When things get rough you need new stuff William Lipps Analytical & Measuring Instrument Division July, 2015

3. It’s out there

4. All methods begin with an idea It’s coming to get you

5. Are you ready?

6. How will you detect it, and are your methods fast enough?

8. MALDI-TOF Mass Spectrometry can identify sub-species in under 2 minutes

9. Red lines indicating a spectrum match

10. Algae is toxic and it Kills

11. LCMSMS Analysis of Microcystins

12. Polar pesticides and herbicides are difficult by GC

13. Analyze pesticides and herbicides by LCMSMS, with very little sample prep

15. We can analyze hundreds more pesticides with high selectivity and sensitivity by GCMSMS

16. Methods can retain full scan capability and add MRM for greater selectivity and sensitivity Scan Chromatogram MRM Chromatogram 13.5013.7514.0014.25 0.25 0.50 0.75 1.00 (x10,000) 279.00>204.90 279.00>222.90 050100150200250300350400 0 25 50 75 100 % 97 223 162 65 279 117 251 173 59 135 203 259 341 397 365305 Qualification Quantification

17. Analysis of solid waste for metals requires acid digestion and instrumental analysis,

18. Or,

19. And create new methods that use XRF X-ray tube Primary X-rays Horizontal axis: Energy (keV) = Type of element Vertical axis: X-ray intensity (cps/uA) = Concentration (ppm) PbCd Cr Al Pb Cr Cd 23.110 kev 10.552 keV 5.412 keV 1.487 keV Al Cr Pb Cd – cps/uA Fluorescent X-rays Detector Analysis sample

20. 20 / 9 20 An example of a whole rock quantitative analysis of cement Na-S SrK-Zn Peak Profile of Cement Standard Substance Sample Appearance (Press-formed at 250 kN for 30 sec) % MgO% Al 2 O 3 % SO 3 % CaO XRF1.753.9521.969.6 CRM1.933.8822.467.9

21. Just place liquids, slurry samples, and emulsions in a sample cell Sample cell, film, and 5 mL oil

22. Elements in Waste Oil (0, 10, 30, and 50 ppm of each element) Overlaid Profiles of Heavy Elements in Waste Oil 30 ppm Blank Sample 50 ppm 10 ppm

23. Foreign Matter Adhering to a Plastic Extruded Part — Fe Kb — Ni Ka — Cr Ka — Cr Ka Mn Kb — Ti Ka — Ti Kb — Ni Kb — Zn Ka — Zn Kb — Mo Ka Foreign matter Normal position Overlaid Profiles of Foreign Matter (Red) and Normal Position (Blue) — Fe Ka Sample Appearance Red circle: Foreign matter Blue circle: Normal position

24. Let’s reduce toxic chemicals

25. Replace toxic cadmium with reductase for NOx Sample #Commercial Laboratory Results, Analysis Method EPA 335.2 (mg NO 3 +NO 2 -N/L) Analysis by Reductase (mg NO 3 +NO 2 -N/L) 10.80.94 2<0.10.05 3<0.10.24 40.660.68 511.811.6 60.780.79 72.43.11

26. Should we continue with the Kjeldahl Method for TKN?

27. Or use HTCO methods and obtain near equal results with TKN

28. Technology is advancing faster than the methods

29. It’s time for some new stuff MALDI TOF – New, faster way to confirm bacteria LCMSMS – Emerging Contaminants not visible before GCMSMS – more sensitive, higher throughput XRF – no digestions, higher throughput NOx – safer, non toxic reagents TN – safer and higher throughput than TKN

30. Thank You, for more information contact me wclipps@shimadzu.com