1. Information in Biology

2. Outline What is synthetic biology? Biological Clocks The “Repressilator” Signal transduction The “Diverter”

3. Outline What is synthetic biology? Biological Clocks The “Repressilator” Signal transduction The “Diverter”

4. Outline What is synthetic biology? Biological Clocks The “Repressilator” Signal transduction The “Diverter”

5. What is synthetic biology?  The design and construction of new biological parts, devices, and systems  The “Repressilator”  The re-design of existing, natural biological systems for useful purposes.  The “Diverter”

8. Outline What is synthetic biology? Biological Clocks The “Repressilator” Signal transduction The “Diverter”

9. Biological - Clocks  Three questions 1. How does it work 2. How does it synchronize 3. How does it translate to activity

10. Biological - Clocks

13. Outline What is synthetic biology? Biological Clocks The “Repressilator” Signal transduction The “Diverter”

14. The “Repressilator”

15. Repressilator  oscillations are favored by Strong promoters Efficient ribosome-binding sites Tight transcriptional repression Comparable protein and mRNA decay rates

16. Repressilator

18. Outline What is synthetic biology? Biological Clocks The “Repressilator” Signal transduction The “Diverter”

19. Signal Transduction

20. The Yeast system  Scaffold proteins  Mediating recruitment  Improve efficiency of signal transfer.  Facilitate interactions among different signal pathways  Control localization of signal proteins within a cell.

21. Outline What is synthetic biology? Biological Clocks The “Repressilator” Signal transduction The “Diverter”

22. The Pre-Diverter

23. The Diverter

24. Diverter results

26. Summary What is synthetic biology?  The design and construction of new biological parts, devices, and systems The Repressilator  The re-design of existing, natural biological systems for useful purposes. The Diverter

27. Signal transduction movies signal transduction  http://www.youtube.com/watch?v=tMMr TRnFdI4 http://www.youtube.com/watch?v=tMMr TRnFdI4 signal transduction  http://www.rpi.edu/dept/bcbp/molbioche m/MBWeb/mb1/part2/signals.htm#anim at2