1. Chapter 8: Sampling, Standardization & Calibration
CHE 321: Quantitative Chemical Analysis
Dr. Jerome Williams, Ph.D.
Saint Leo University

2. Overview
Standardization & Calibration
Sensitivity & Detection Limits

3. Standardization & Calibration
Calibration determines relationship between analyte response & analyte concentration.
Accomplished using chemical standards.

4. Standardization & Calibration
Comparison with Standards
Direct comparison (Colorimeters & Titrations)
External Standard Calibration (Least Squares Method)

5. Figure 8-9 p172

6. Figure 8-10 p172

7. Table 8-1 p175

8. Table 8-2 p177

9. Table 8-3 p178

10. Fig 8-9, p.195

17. Standardization & Calibration
Errors in External Standard Calibration
Blanks are used to correct for many calibration errors.
Ideal blank contains everything except the analyte.
Solvent blank or reagent blank more practical.

18. Standardization & Calibration
Minimizing Errors in Analytical Procedures
Multivariate Calibration
Saturation, Matrix Modification, & Masking
Internal Standard Methods
Standard Addition Methods

19. Sensitivity & Detection Limits
Be familiar with the following terms
Analytical & Calibration Sensitivity
Detection Limit
Linear Dynamic Range
Control Chart (including UCL, LCL)
Statistical Control
Validation & GLP

20. Figure 8-14 p187

21. Figure 8-15 p189

22. p189

23. p189

24. Figure 8-16 p190

25. Suggested Problems
HW Set 7: 8.1, 8.2, 8.3, 8.17, 8.26