1. LABORATORY SAFETY

2. Before This Lab Course Your Instructor will:  Inform you of YOUR responsibilities for working in the labs  Show you where and how to dispose of the chemicals and other materials that you will be working with.  Show you where the M.S.D.S. files are located  Be informed of any accident or incident that occurs in the laboratory

3.  Show you the location of and how to use all safety equipment needed  Including:  Safety Shower and Eyewash  Spill Kit  Emergency Gas Shutoff  Fire Extinguisher & Fire Blanket  Lab Phone with 911 Information  Chemical Hygiene Plan w/ Emergency Phone numbers Before This Lab Course Your Instructor will:

4. Also Get to Know: [ Emergency spill procedures, use of absorbents and disinfectants [ Designated escape route and alternate routes [ Evacuation Procedures

5. While Working in the Lab: The following rules can be found in the Chemical Hygiene Plan [ Glass / Glass Tubing/ Thermometers. Use glassware for its designated purpose. When inserting any glass into stoppers use a 'safety grip' and lubricant to help prevent breaking. Wear goggles and gloves and also use proper tools for cutting glass tubing. [ No Smoking. This policy exists throughout the College and applies in all laboratories. [ Housekeeping. Exits, aisles and safety equipment must NOT be obstructed in any way with equipment, furniture, etc. Items must not be stored in the corridors

6. While Working in the Lab: The following rules can be found in the Chemical Hygiene Plan [ Food, Drink, Cosmetics. Eating, drinking and the application of cosmetics (including lip balm) is forbidden in areas where hazardous chemicals are used and must be done only in well-defined designated non-chemical areas. Do not store food in the same refrigerator with chemicals, biohazards or radioactive materials. [ Horseplay. Horseplay, practical jokes or other inappropriate and unprofessional behavior in the laboratory setting is forbidden. Avoid distracting or startling any other students/ workers

7. While Working in the Lab: The following rules can be found in the Chemical Hygiene Plan [ Equipment. Use proper equipment that is in good condition, i.e. never use chipped or cracked glassware inform your instructor of any equipment that is broken or possibly dangerous. Shield pressurized or vacuum apparatus and safeguard boiling liquids against bumping or overheating. Use safeguards whenever possible [ Handling of Chemicals. Do not taste or smell chemicals. Use chemicals for which the quality of available ventilation and other protection is appropriate. If unsure consult MSDS prior to beginning work.

8. While Working in the Lab: The following rules can be found in the Chemical Hygiene Plan [ Hazardous Materials. Hazardous materials should not be used on open lab benches and work should not be done alone. When working with acutely hazardous materials over an extended period or working with any material that has a rating of 4 in any one of the NFPA Diamonds you must refer to App. IV of the Chemical Hygiene Plan [ Mouth Pipetting. Mouth pipetting is forbidden.

9. While Working in the Lab: The following rules can be found in the Chemical Hygiene Plan [ Proper Apparel : Wear splash-proof goggles; face shield, etc. Wear a lab coat or apron if possible; at a minimum attire should include sleeves as well as cover the full length of your legs; close-toed shoes must be worn in the lab. Confine long hair and loose clothing or jewelry. False fingernails are not recommended, as they are flammable. [ Personal Hygiene: Do not put your hands in your eyes or mouth. Make sure your hands are washed before leaving the laboratory area.

10. While Working in the Lab: The following rules can be found in the Chemical Hygiene Plan [ Disposal of Wastes. It is important to segregate wastes. Disposal of all laboratory waste must follow prudent procedures for the class of waste being disposed of or properly stored until disposal can be arranged. See the chart Posted in each Lab [ Know the Locations of the Biohazard Waste containers and Sharps Containers

11. Eye protection  Protects against risk of flying objects or dust particles, splashes of hazardous materials or harmful rays  UV Goggles are available

12. Safety Glasses  Unbreakable lenses of plastic or tempered glass  For light-to-moderate work  Can be prescription lenses  Do not interfere with contact lenses

13. Goggles  Work with significant risk of splash of chemicals or projectiles  Can be worn over prescription glasses

14. Face Shield  Work with significant risk of splash on face or possible explosion  Face shield protects face adequately but not eyes

15. Hand Protection 4 Protects against risk of cuts, abrasions, burns, or exposure to hazardous materials. 4 Requires selection of the appropriate chemical resistant gloves ** see chart in Chemical Hygiene Plan

16. While Working in the Lab: [ Follow universal precautions, for any suspected biological hazard. [ Handle unknowns as if they were hazardous

17. While Working in the Lab: [ Handle volatiles in a chemical fume hood Contain bioaerosols in a biological safety cabinet

18. While Working in the Lab: [ Authorized persons only no students without an Instructor present [ Identify EVERYTHING! ALL containers should be clearly labeled with their contents

19. Report all: [ Accidents [ Injuries [ Fires [ Spills [ Close calls While Working in the Lab:

20. Before Leaving the Lab: Turn off: [ Gas [ Water [ Power supplies [ Vacuum lines [ Compression lines [ Heating apparatus

21. Before Leaving the Lab: [ Identify and package waste, dispose properly [ Identify and label defective equipment [ Decontaminate work surfaces and equipment

22. Before Leaving the Lab: [ Return unused equipment, apparatus, etc. [ Leave lab coat in the lab [ Wash [ Close and lock door