1. Supplementary Figure 1

2. Supplementary Figure 2

3. Suppl. Fig. 1. The promoter of miR-155 encoding Bic gene contains two putative NF-  B binding sites. It is drown a partial nucleotide sequence of Bic promoter (from + 150 to – 222; from – 1064 to – 1912) containing the putative cis elements with higher score. The TATA box (-25 in pink), the AP1 site (-40 in red) the SP1 site (- 97 in blue), the two NF-  B binding sites (-1150; -1697 in green), and the PU.1 site ( -1403 in purple). Suppl Fig. 2. Inhibition of NF-  B canonical pathway leads to a reduction of miR-155 levels in EBV immortalized MC3 cells. A) Western Blot analysis of total protein extracts obtained from MC3 untransfected (MC3 UT), MC3 transfected with pRc/CMV-HA-I  B  -S32/36A (MC3 I  B  and MC3 transfected with Empty Vector (MC3 EV). The blot was analyzed for I  B  expression. The exogenous protein runs higher than the endgenous because has HA- FLAG. B) Western Blot analysis of nuclear extracts obtained from MC3 untransfected (MC3 UT), MC3 transfected with pRc/CMV-HA-I  B  -S32/36A (MC3 I  B  and MC3 transfected with Empty Vector (MC3 EV). The blot was analyzed for p65 expression and normalized by H3. C) Graphic representation of the Western blot showed in B results as average of three independent experiments. The nuclear p65 decreases when the undegradable form of I  B  is expressed. D) Northern Blot analysis of total RNAs obtained from MC3 untransfected (MC3 UT), MC3 transfected with pRc/CMV-HA-I  B  -S32/36A (MC3 I  B  and MC3 transfected with Empty Vector (MC3 EV). The blot was analyzed for miR-155 expression and normalized by U6. E) Graphic representation of the Northern blot results as average of three independent experiments.