Download presentation
Presentation is loading. Please wait.
Published byDuane Mills Modified over 8 years ago
1
Prion Digestion in the Alimentary Tract Nicholas Toney
2
Overview Outline of the Digestive Tract Dr. Manuelidis’s Claims Experimental Procedure Discussion
3
Ruminant Digestion
4
Digestion
5
Claim to Brain Manuelidis’s Claim: All detectable forms of PrP are digested in the gastro intestinal tract yet the invasive infectious particle (as many conventional viruses) is not destroyed
6
Transportation of prion protein across the intestinal mucosa of scrapie-susceptible and scrapie-resistant sheep Experimental Methods and Goals In Vivo GI inoculation In Vitro GI digestion of infectious brain slurry
7
Western Blot
8
Infectivity of Scrapie Prion Protein (PrPSc) Following In vitro Digestion with Bovine Gastrointestinal Microbiota Goals Methodology In Vitro Digestion In Vivo determined level of infectivity
9
Discussion Acid Resistance of Prions, digestion by microbiota What limitations are there with the experimental methods? Are the in-vitro digestion experiments a good model of digestion? How do you reconcile the loss of detectable PrPres with the continued infectivity of brain slurry from Scrapie infected brain material?
Similar presentations
© 2024 SlidePlayer.com. Inc.
All rights reserved.