1. TAXONOMY Group:Group V ((-)ssRNA) Order:Mononegavirales
Family:Rhabdoviridae
Genus: Lyssavirus
TYPE SPECIES: Rabies virus
Species
Aravan virus Australian bat lyssavirus Duvenhage virus European bat lyssavirus 1 European bat lyssavirus 2 Irkut virus Khujand virus Lagos bat virus Mokola virus West Caucasian bat virus

2. MORPHOLOGY RIGID BULLET-SHAPED -VE SENSE NON SEGMENTED
SINGLE-STRANDED RNA VIRUS
ENVELOPE

5. MULTIPLICATION Similar to other negative stranded RNA viruses
Attach to host cell via G-protein
Penetrate cytoplasm by fussion or pinocytosis
Uncoated to RNP
Core initiates primary transcription of 5 complementary monocistronic messenger RNA
Synthesis of viral protein
Replication of genomic RNA
Synthesis of positive stranded RNA
Production of negative stranded RNA

6. CULTIVATION All warm blooded animals
Rabbits, guinea pigs, rats and mice are useful for primary isolation
Chick and duck embryo
In the range of all cell cultures, including baby hamster kidney and mouse neuroblastoma cells, human diploid lung fibroblasts, chick embryo fibroblasts
Vero monkey kidney cells, through with minimal cytopathic effects.

7. RESISTANCE Ehanol,iodine preparation Quaternary ammonium compounds
Lipid solvent,eg:ether, chloroform and acetone
Phenol, formalin, beta-propiolactone
Proteolytic enzymes
UV irritation
Sunlight
Thermal:50°C for 1 hour,60°C for 5 min

8. ANTIGENIC STRUCTURE
Single immunologic type

9. EPIDEMIOLOGY classic zoonotic infection,
spread from animals(dogs, cats, foxes, bats, etc.) to human
recognized for over 4,000 years.
attributable to a bite (any penetration of the skin by the teeth)
secretion of the virus in the animal’s saliva and promote the transmission of the virus.
Nonbite exposures eg:contamination of an open wound or a mucous membrane via scratches, licks, and inhalation of aerosol, in transplanted infected tissue and by inoculation through intact mucosal membranes

11. PATHOGENESIS

12. CLINICAL SYMPTOMS 5 general stages: -incubation -prodrome
-acute neurologic period
-coma
-death (or, very rarely, recovery).

13. LABORATORY DIAGNOSIS diagnosis(postmortem)
microscopical, virological, and serological method
aggregates of viral nucleocapsids(Negri bodies).
The detection of rabies antigen, antibody, viral RNA, or the isolation of virus establishes a diagnosis of rabies.
direct immunofluorescence testing
ELISA