1. © Elsevier, 2011.Principles of Molecular Virology Virus Replication How studying bacteriophages has helped understanding of virus replication Virus replication, step by step

2. © Elsevier, 2011.Principles of Molecular Virology Investigation of Virus Replication Ellis and Delbruck, 1939 - "single-burst" experiment: Initiation of infection Replication and expression of the virus genome Release of mature virions from the infected cell

3. © Elsevier, 2011.Principles of Molecular Virology Investigation of Virus Replication

4. © Elsevier, 2011.Principles of Molecular Virology Other viruses, e.g. picornaviruses

5. © Elsevier, 2011.Principles of Molecular Virology Other viruses, e.g. picornaviruses

6. © Elsevier, 2011.Principles of Molecular Virology Hershey and Chase, 1952

7. © Elsevier, 2011.Principles of Molecular Virology The Virus Replication Cycle

8. © Elsevier, 2011.Principles of Molecular Virology Attachment - Picornavirus

9. © Elsevier, 2011.Principles of Molecular Virology Attachment - Influenza Virus

10. © Elsevier, 2011.Principles of Molecular Virology Penetration

11. © Elsevier, 2011.Principles of Molecular Virology Uncoating

12. © Elsevier, 2011.Principles of Molecular Virology Genome Replication and Gene Expression Class I: Double-stranded DNA:

13. © Elsevier, 2011.Principles of Molecular Virology Class II: Single-stranded DNA

14. © Elsevier, 2011.Principles of Molecular Virology Class III: Double-stranded RNA

15. © Elsevier, 2011.Principles of Molecular Virology Class IV: Single-stranded (+)sense RNA

16. © Elsevier, 2011.Principles of Molecular Virology Single-stranded (–)sense RNA

17. © Elsevier, 2011.Principles of Molecular Virology Single-stranded (+)sense RNA with DNA intermediate

18. © Elsevier, 2011.Principles of Molecular Virology Double-stranded DNA with RNA intermediate

19. © Elsevier, 2011.Principles of Molecular Virology Assembly | Maturation

20. © Elsevier, 2011.Principles of Molecular Virology Release Release

21. © Elsevier, 2011.Principles of Molecular Virology Summary Virus replication involves three broad stages: Initiation of infection Replication and expression of the genome Release of mature virions from the infected cell

22. © Elsevier, 2011.Principles of Molecular Virology Further Reading Cann, A.J. (2000) DNA Virus Replication: Frontiers in Molecular Biology. Oxford University Press, Oxford. ISBN 019963713X Ellis, E.L. and Delbruck, M. (1939) The growth of bacteriophage. Journal of General Physiology, 22: 365–384 Freed, E.O. (2004). HIV-1 and the host cell: an intimate association. Trends in Microbiology, 12: 170–177 Gonzalez, M.E. and Carrasco, L. (2003) Viroporins. FEBS Lett. 552 (1): 28-34 Hershey, A.D. and Chase, M. (1952). Independent functions of viral protein and nucleic acid in growth of bacteriophage. Journal of General Physiology, 26: 36–56 Kasamatsu, H. and Nakanishi, A. (1998) How do animal DNA viruses get to the nucleus? Annual Review of Microbiology, 52: 627–686 Lopez, S. and Arias, C.F. (2004) Multistep entry of rotavirus into cells: a Versaillesque dance. Trends in Microbiology, 12: 271–278 Moore, J.P. et al. (2004) The CCR5 and CXCR4 coreceptors - central to understanding the transmission and pathogenesis of human immunodeficiency virus type 1 infection. AIDS Research and Human Retroviruses, 20: 111–126 Rossmann, M.G. et al. (2002) Picornavirus–receptor interactions. Trends in Microbiology, 10: 324–331 Sattentau, Q. (2008) Avoiding the void: cell-to-cell spread of human viruses. Nat Rev Microbiol. 6 (11): 815-826 Schneider-Schaulies, J. (2000) Cellular receptors for viruses: links to tropism and pathogenesis. Journal of General Virology, 81: 1413–1429 Welsch, S., Müller, B. and Kräusslich, H.G. (2007) More than one door - Budding of enveloped viruses through cellular membranes. FEBS Lett. 581 (11): 2089-2097