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Observing and Measuring Cells Lab
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1. Measure Using Reticule
Reticule- ruler within the ocular lens Use to measure specimen observing under the microscope Length Reticule Measurement (400x):_________ Width Reticule Measurement (400x):________
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2. Use the conversion table provided to determine µm
Reticule Measurement:_________ How to use the table! Get the # of A, B, & C’s to add up to your total reticule measurement Example: Reticule Meas. = 1.6 1 C + 1 B + 1 A C 1 A B Objective Lens Power Microscope Magnification A B C Scanning 40x 25um 125 um 250 um Low 100x 10um 50 um 100 um High 400x 2.5um 12.5 um 25 um Oil 1000x 1um 5 um 10 um
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3. Convert to mm (life size!)
Bigger- move decimal point to the left Unit Deci 0.1 (d) Centi 0.01 (c) Milli 0.001 (m) Decimilli 0.0001 (dm) Centimilli (cm) Micro (µ) Smaller- move decimal point to the right Multiply um by _________________ to get mm, or move the decimal place over ____ spaces. Table Converted Reticule Length:___________ This gives you the Life Size of the object
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Calculating Drawing Magnification (Length Only!)
100 ÷largest life size Example: 100 ÷ __________ = Round to workable # __________________ Written as _____________ x L.S.
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Calculate Drawing Dimensions
Life size x drawing magnification ____________ x ____________ These are the dimensions that you will draw Include labels (follow the rubric)
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Width Calculations Only determine life size
Do NOT calculate magnification Use the magnification from length
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Cell Drawing
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Cell Drawing Within 100mm x 100 mm box, measure and draw the length & width of the object (mm) Include organelles you could see under the microscope Follow rubric provided
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