1. tRNA intron endonuclease : The case of the missing tRNA-trp Peter Bakke

2. The Search: RAST found endonuclease found tRNA-trp

3. pre-tRNA H. volcanii Northern blot

4. generic pre-tRNA:pre-tRNA-trp: Bulge-helix- bulge motif 104-bp intron

5. pre-tRNA H. volcanii Northern blot circular intron linear intron tRNA 3’ exon 5’ exon

6. References: Thompson, “A tRNA-trp intron endonuclease from Halobacterium volcanii”, Journal of Biological Chemistry 1988. Thompson, “Recognition of exon-intron boundaries by the Halobacterium volcanii tRNA intron endonuclease”, Journal of Biological Chemistry 1990. Tocchini-Valentini et al, “Structure, function, and evolution of the tRNA endonucleases of Archaea”, PNAS 2005. Trotta, “Cleavage of pre-tRNAs by the splicing endonuclease requires a composite active site”, Nature 2006. Clouet d’Orval, “Box C/D RNA guides for the ribose methylation of archaeal tRNAs”, Nucleic Acids Research 2001. Gupta Lab: http://www.siumed.edu/~rgupta/Research.html