1. L-Lysin-Production with Corynebacterium glutamicum Lothar Eggeling & Michael Bott ; Christoph Wittmann By judy mutua

2. Outline  Introduction  Methods  Results  Conclusion

3. Introduction  One of essential amino acids  Feed & Food additive  Medicine  chemicals  2,200,000 tons/year

4. Introduction

6. Method

7. New screening method

8. Method  Fed-batch fermentation  Quantification of substrates and products  Quantification of enzyme activity  Metabolic modeling and parameter estimation for 13 C metabolic flux analysis  Elementary flux mode analysis

9. Results

11. Conclusion  Metabolic engineering is a strategy for creation of tailor made strains.  Little investigation on +ve mutations.  In fedbatch process growth is inhibited.  A concerted interaction is necessary.