1. Microbiology B.E Pruitt & Jane J. Stein AN INTRODUCTION EIGHTH EDITION TORTORA FUNKE CASE Chapter 6, part B Microbial Growth

2. Deep-freezing: -50°to -95°C Lyophilization (freeze-drying): Frozen (- 54° to -72°C) and dehydrated in a vacuum Preserving Bacteria Cultures

3. Binary fission Budding Conidiospores (actinomycetes) Fragmentation of filaments Reproduction in Prokaryotes

4. Binary Fission Figure 6.11

5. Figure 6.12b

6. If 100 cells growing for 5 hours produced 1,720,320 cells:

7. Figure 6.13

8. Figure 6.14

9. Plate Counts: Perform serial dilutions of a sample Direct Measurements of Microbial Growth Figure 6.15, top portion

10. Inoculate Petri plates from serial dilutions Plate Count Figure 6.16

11. After incubation, count colonies on plates that have 25-250 colonies (CFUs) Plate Count Figure 6.15

12. Filtration Direct Measurements of Microbial Growth Figure 6.17a, b

13. Multiple tube MPN test Count positive tubes and compare to statistical MPN table. Direct Measurements of Microbial Growth Figure 6.18b

14. Direct Measurements of Microbial Growth Direct Microscopic Count

15. Direct Measurements of Microbial Growth Figure 6.19

16. Turbidity Estimating Bacterial Numbers by Indirect Methods Figure 620

17. Estimating Bacterial Numbers by Indirect methods Metabolic activity Dry weight