1. Michalska, ATVB, 2012, online

2. http://www.clinchem.org/content/54/1/24/F1.expansion.html Endothelial Cells Leukocyte Intima Smooth Muscle Cells VCAM (on EC)

3. USPIO http://nihlibrary.ors.nih.gov/ipdcdb/ipdcdb_main.asp?Page=4 VCAM- binding peptide P03011 (Contrast Agent for MRI) (targeting motif)

4. SECARS? Light hits molecule Molecule energy is shifted up to virtual energy state Photon emitted when molecule drops down in energy Anti-Stokes is when energy is less than original Allows for high resolution imaging http://en.wikipedia.org/wiki/Raman_spectroscopy

5. t 1/2 = 4.0±2.4h t 1/2 = 5.0±1.7h t 1/2 = 7.2±2.5h t 1/2 = 6.0±1.3h

6. Prussian blue stains iron Spleen pre- injection Liver pre- injection Spleen post- injection Liver post- injection Lymph post- injection kidney post- injection muscle post- injection Lung post- injection DAPI CD68

7. Michalska, ATVB, 2012, online

8. 12 wk 30 wk

9. Michalska, ATVB, 2012, online

10. early plaques late plaques Control injection

12. Michalska, ATVB, 2012, online

14. Chen, Biomaterials, 2009, 30, 79-88