1. Synthetic Biology at Davidson College Building Bacterial Computers

2. Advantages of Bacterial Computation $ ¢ Computation Hardware Software Computation

3. Advantages of Biological Computers go anywhere - arctic, thermal vents, inside organisms no electricity self-replicating no immune rejection

4. Self-replicating Computers

5. Two Recent Research Projects

6. Outstanding Publication of 2008 in the Journal of Biological Engineering 12 undergraduate coauthors

7. Flipping DNA with Hin/hixC

10. 14 2 53 Hamiltonian Path Problem

11. 14 2 53 Hamiltonian Path Solution

12. Engineering Biological HPP

16. Hin-mediated recombination

17. Engineering Biological HPP

18. Predicting Outcomes of Bacterial Computing

19. Does Starting Place Matter?

20. Equilibrium Does Starting Place Matter?

21. How Many Plasmids Do We Need?

22. Split Genes to Encode Problem

23. gcat.davidson.edu/GcatWiki/index.php/Davidson_Missouri_W/Davidson_Proto cols

24. Split Reporter Genes Green Fluorescent Protein Red Fluorescent Protein

25. Split Reporter Genes RFP Split by hixC GFP Split by hixC

26. Bacteria Start Computation Yellow colonies indicate solution found Unflipped Flipped

27. Bacteria Report Solutions Yellow colonies indicate solution found Unflipped Flipped

28. Bacteria Report Solutions Yellow colonies indicate solution found Unflipped Flipped

29. Paper Published 7/09 15 undergraduate coauthors Paper of the year, 2009

30. Can we solve the SATisfiability problem?

31. Define the SATisfiability Problem

34. Converting Math to Biology

35. Central Dogma

36. Frameshift Mutation

37. Frameshift Suppression

38. Suppressor tRNA

39. Coding 2-SAT Clause

42. Outcomes of v 1.0

44. Redesign System v2.0 positive feedback loop

45. Outcomes of v 2.0 - control+ control

46. Outcomes of v 2.0 - control frame shift “leak” +tRNA CGGUC +tRNA CCACU + control

47. Outcomes of v 2.0 frame shift “leak” +tRNA CGGUC +tRNA CCACU + control

48. Why build bacterial computers?

49. Evolution of Computers 1943

50. iPhone in 2011 Evolution of Computers

51. E. coli in 2011 ? Living Hardware in 2021 Evolution of Bacterial Computers