1. Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 1 Prepare a Wet Mount CAPT Mike Hughey, MC, USNR

2. Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 2 First, a Video

3. Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 3 Obtain a Specimen Spatula Cotton-tipped Applicator Obtain directly or from inside curve of speculum

4. Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 4 Place on Microscope Slide Use very small amount Too much makes the slide too thick and unreadable

5. Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 5 Add NaCl and KOH One drop Mix well Two slides vs. one slide?

6. Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 6 Add Coverslip Remove excess fluid so the coverslip is stable. Heat the KOH slide?

7. Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 7 Microscopic Examination Start with low power Rarely need to go above medium power Focus up and down through the plane of the cells

8. Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 8 Yeast Tangle of threads Single branch

9. Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 9 Watch this video

10. Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 10 Trichomonas About the same size as a white blood cell Smaller than a vaginal epithelial cell Violent motion

11. Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 11 Watch this video

12. Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 12 Bacterial Vaginosis Clue cells Vaginal epithelial cells studded with bacteria Smudged, granular appearance Indistinct borders Clue Cell Normal Vaginal Epithelial Cell

13. Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 13 Clue Cells

14. Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 14