2. 1st week2nd week

4. Protein G Sepharose High Performance is produced by coupling protein G to highly cross-linked agarose beads by the N-hydroxysuccinimide activation method. Amersham Biosciences recombinant protein G, Mr 17 000, is produced in E. coli and contains two IgG binding regions. The albumin binding region of native protein G has been genetically deleted, thereby avoiding undesirable cross-reactions with albumin.

6. equilibration Specific binding and wash out Elution Regeneration (equilibration)

11. A B UV detector (280 nm) Buffers Columns 1.Protein G 2.DEAE Pump Tube no. OD 280 123456 78910

12. Protein G DEAE SerumMilk 37 4 5 8 9 Gel slab + 34567812910 __ + 26 37 std 1st week2nd week 3rd week