1. Systemic Onset Juvenile Rheumatoid Arthritis (SOJRA) Children present with spiking fevers, rash, enlarged liver / spleen, lymphadenopathy, systemic inflammation, and arthritis 50 % Remission

2. Predictor discovery in training set 1 sample set A (11 F, 10 Q) PAM Classifier training analysis Cross-validation Biomarker panels Study Design Gaussian LDA Class prediction in testing set 3 sample set B (10 F, 10 Q) PAM Class prediction ROC curve analysis Power analysis Sample size estimation 2 Select optimum panel Unsupervised clustering

3. Figure 4 Training AUC = 0.97 SJIA F Q classifier 29 feature panel Testing AUC = 0.73 Sensitivity 1- Specificity 0.0 0.2 0.4 0.6 0.8 1.0 0.0 0.2 0.4 0.6 0.8 1.0 4 8 12 19 29 3963122174266 -0.5 0.0 0.5 1.0 Goodness of class separation –  probability Feature# * Flare Quiescent A B

4. Sample set A + B n = 41 2120 Clinical diagnosis FQ n = 173 4 Clustered as F Clustered as Q Percent Agreement with clinical diagnosis 81% 85% + - 83% Overall P = 3 X 10 -5 SJIA FSJIA Q 2d cluster *** * SJIA Q with impending F * SJIA F with impending Q

5. F F (low)QF 11/0612/0602/0704/07 Diagnosis Clinical visit date Peptide signal ESR JC Ferritin CRP N.D.

6. 11/0612/0602/0704/07 Clinical visit date F F (low)QF Diagnosis PO pred Peptide Signal

7. 11/0612/0602/0704/07 F F (low)QF Diagnosis Clinical visit date WBC PLT Peptide signal

8. 11/0612/0602/0704/07 Clinical visit date F F (low)QF Diagnosis Peptide Signal MTX dose